The procedure for the induction of DMBA-induced mammary lesions has been described in detail previously (Telang et al., PNAS 76:5886, 1979; Mehta and Moon, Cancer Res. 46:5832, 1986). BALB/c female mice obtained are used in the study. The animals are pretreated subcutaneously with 1 g 17 -estradiol + 1 mg progesterone for 9 days. The thoracic mammary glands are excised and incubated in Waymouth's MB752 medium with 5 g insulin, 5 g prolactin, 1 g aldosterone and 1 g hydrocortisone per ml medium for 10 days with a 24 hour exposure of the glands to DMBA (2 g/ml) on day 3. The glands are then cultured for 14 days in the presence of 5 g/ml insulin alone. The other experimental conditions include 37xC temperature, 50% O2, 5% CO2, and 45% N2 atmosphere. Two control groups are included in each test, a DMSO and a DMBA Control. The chemopreventive agents are included in the medium for the first 10 days of culture at 5 different concentrations ranging from 1 x 10-9M to 1 x 10-5M. At the end of 24 day experiment, the glands are fixed in formalin, stained with alum carmine and evaluated for the incidence and multiplicity of the mammary lesions in these glands. There are 25 chemopreventive agents to be evaluated by this assay.

Agency
National Institute of Health (NIH)
Institute
Division of Cancer Prevention And Control (NCI)
Type
Research and Development Contracts (N01)
Project #
N01CN095102-000
Application #
6129525
Study Section
Project Start
1999-06-30
Project End
Budget Start
1999-06-30
Budget End
2000-12-30
Support Year
Fiscal Year
1999
Total Cost
Indirect Cost
Name
University of Illinois at Chicago
Department
Surgery
Type
Schools of Medicine
DUNS #
121911077
City
Chicago
State
IL
Country
United States
Zip Code
60612