We propose to develop a complete set of sub-chromosomal fluorescence in situ hybridization (FISH) probes for the mouse genome. The probes will be based on both chromosomal microdissection and YAC clones containing large mouse genomic inserts. The microdissection-based probes will be used for spectral banding, a multi-color single chromosome FISH technique to analyze all types of sub-chromosomal rearrangements. Because the spectral banding probe sets (up to 7 probes per chromosome) will be keyed to the standard Giemsa banding pattern, users will be able to describe their findings in terms of conventional G-banding momenclature. A YAC-based set of linkage-cytogenetic FISH probes spaced at about 5 cM intervals, will be developed to coordinate a cytogenetic band-anchored linkage map for the mouse. We also propose to build a set of spectral banding probes for the human karyotype and to use the mouse linkage-cytogenetic probes to coordinate human/mouse co-maps. This interspecies coordination of cytogenetic maps, when used with cross-species FISH, will allow researchers using other mammalian species to have access to the rich human and mouse gene databases, thus widening the commercial market for these probes.

Project Start
1997-09-04
Project End
Budget Start
1998-09-18
Budget End
1999-09-01
Support Year
Fiscal Year
1998
Total Cost
Indirect Cost
Name
Applied Genetics Laboratories, Inc.
Department
Type
DUNS #
City
Melbourne
State
FL
Country
United States
Zip Code
32904