Abstract

This program is directed towards a better understanding of the factors which regulate normal hematopoiesis in animals and man. We will investigate the role of cellular elements and their humoral products which regulate the self replication, proliferation, and maturation of hematopoietic progenitor cells. Utilizing murine models including the W/Wv and S1/S1d anemic mouse models, congenic mouse strains, and normal mice as sources of bone marrow and peripheral blood cells, we will attempt to identify and characterize specific subpopulations of cells responsible for the regulation of hematopoietic cell proliferation. We will also evaluate the appearance of these subpopulations in ontogeny. The subpopulations will be evaluated utilizing co-culture techniques and conditioned media production in order to determine their mechanism of action (soluble factors versus direct cell-cell interactions). We will evaluate the role of such regulatory cells and soluble factors in in vitro clonal assay systems (CFU-c, BFU-c and CFU-e) as well as in diffusion chamber assays and long-term continuous bone marrow cultures. Similar studies will be carried out with normal and perturbed human bone marrow and populations from the peripheral blood and bone marrow of normal human volunteers.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis, Diabetes, Digestive and Kidney Diseases (NIADDK)
Type
Research Program Projects (P01)
Project #
5P01AM027157-07
Application #
3092265
Study Section
(SRC)
Project Start
1980-09-30
Project End
1987-02-28
Budget Start
1985-09-01
Budget End
1987-02-28
Support Year
7
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Johns Hopkins University
Department
Type
Schools of Medicine
DUNS #
045911138
City
Baltimore
State
MD
Country
United States
Zip Code
21218

  Publications

Sieber, F; Krueger, G J; O'Brien, J M et al. (1989) Inactivation of Friend erythroleukemia virus and Friend virus-transformed cells by merocyanine 540-mediated photosensitization. Blood 73:345-50
La Russa, V F; Sensenbrenner, L L; Sharkis, S J (1989) Effects of in vivo neuraminidase on the regulation of erythropoiesis. II: Modulation of erythroid colony formation by thymic regulatory cells. Exp Hematol 17:106-9
Kraft, A S; Baker, V V; May, W S (1987) Bryostatin induces changes in protein kinase C location and activity without altering c-myc gene expression in human promyelocytic leukemia cells (HL-60). Oncogene 1:111-8
Rowley, S D; Zuehlsdorf, M; Braine, H G et al. (1987) CFU-GM content of bone marrow graft correlates with time to hematologic reconstitution following autologous bone marrow transplantation with 4-hydroperoxycyclophosphamide-purged bone marrow. Blood 70:271-5
Rowley, S D; Sharkis, S J; Hattenburg, C et al. (1987) Culture from human bone marrow of blast progenitor cells with an extensive proliferative capacity. Blood 69:804-8
Sieber, F; O'Brien, J M; Krueger, G J et al. (1987) Antiviral activity of merocyanine 540. Photochem Photobiol 46:707-11
Sieber, F; Stuart, R K; Rowley, S D et al. (1987) Dye-mediated photolysis of normal and neoplastic hematopoietic cells. Leuk Res 11:43-9
May, W S; Ihle, J N (1986) Affinity isolation of the interleukin-3 surface receptor. Biochem Biophys Res Commun 135:870-9
Strauss, L C; Rowley, S D; La Russa, V F et al. (1986) Antigenic analysis of hematopoiesis. V. Characterization of My-10 antigen expression by normal lymphohematopoietic progenitor cells. Exp Hematol 14:878-86
Stuart, R K; Mangan, K F (1986) Hematologic and cytogenetic remission of 5q-refractory anemia after syngeneic bone marrow transplantation. Am J Med 80:503-7

Showing the most recent 10 out of 17 publications