Abstract

This core will provide a centralized service for producing the transgenic mice initially required in Program Projects 3 and 7. Specifically, the core will undertake the following procedures: (1) microinjection of fertilized one-cell mouse eggs with specific DNA constructs prepared by Program Project investigators, (2) injection of ES cells in blastocysts, (3) return the eggs or blastocysts to pseudopregnant foster mothers, (4) screen for transgenic offspring by Southern analysis of DNA isolated from tails, (5) organize the mating and pedigree analysis of transgenic mice, and (6) assist in the dissection and analysis of tissues from transgenic mice and embryos. Such a facility is not available at present on the Vanderbilt campus. Therefore, in addition to providing an increasingly important research tool for members of the Program Project, it should also catalyze interaction with other members of the Medical School who may wish to establish the technique, or collaborate on projects related to the expression and mode of action of factors regulating growth and differentiation in vivo.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
5P01CA048799-03
Application #
3808011
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
3
Fiscal Year
1991
Total Cost
Indirect Cost

  Institution

Name
Vanderbilt University Medical Center
Department
Type
DUNS #
004413456
City
Nashville
State
TN
Country
United States
Zip Code
37212

  Publications

Satterwhite, D J; White, R L; Aakre, M E et al. (2001) TGF-beta1 regulates the expression of multiple max-interacting transcription factors in Balb/MK cells: implications for understanding the mechanism of action of TGF-beta1. Pediatr Res 50:67-75
Engel, M E; McDonnell, M A; Law, B K et al. (1999) Interdependent SMAD and JNK signaling in transforming growth factor-beta-mediated transcription. J Biol Chem 274:37413-20
Norgaard, P; Law, B; Joseph, H et al. (1999) Treatment with farnesyl-protein transferase inhibitor induces regression of mammary tumors in transforming growth factor (TGF) alpha and TGF alpha/neu transgenic mice by inhibition of mitogenic activity and induction of apoptosis. Clin Cancer Res 5:35-42
Lutterbach, B; Hann, S R (1999) c-Myc transactivation domain-associated kinases: questionable role for map kinases in c-Myc phosphorylation. J Cell Biochem 72:483-91
Gorska, A E; Joseph, H; Derynck, R et al. (1998) Dominant-negative interference of the transforming growth factor beta type II receptor in mammary gland epithelium results in alveolar hyperplasia and differentiation in virgin mice. Cell Growth Differ 9:229-38
Alexandrow, M G; Moses, H L (1998) c-myc-enhanced S phase entry in keratinocytes is associated with positive and negative effects on cyclin-dependent kinases. J Cell Biochem 70:528-42
Zhao, G Q; Liaw, L; Hogan, B L (1998) Bone morphogenetic protein 8A plays a role in the maintenance of spermatogenesis and the integrity of the epididymis. Development 125:1103-12
Engel, M E; Datta, P K; Moses, H L (1998) RhoB is stabilized by transforming growth factor beta and antagonizes transcriptional activation. J Biol Chem 273:9921-6
Datta, P K; Chytil, A; Gorska, A E et al. (1998) Identification of STRAP, a novel WD domain protein in transforming growth factor-beta signaling. J Biol Chem 273:34671-4
Serra, R; Johnson, M; Filvaroff, E H et al. (1997) Expression of a truncated, kinase-defective TGF-beta type II receptor in mouse skeletal tissue promotes terminal chondrocyte differentiation and osteoarthritis. J Cell Biol 139:541-52

Showing the most recent 10 out of 62 publications