The overall goal of the Tissue Analysis Laboratory (""""""""the Core"""""""") is to provide technical and professional support for each Project. The Core at the University of Virginia has been in place for the past decade of the two previously funded prostate POI programs. All assessments will be made by two highly skilled, experienced genitourinary pathologists with expertise in examining mouse and human prostate tissues and in immunohistochemistry. Dr. Frierson will provide overall direction ofthe Core laboratory at the University of Virginia, while Dr. Lucia will lead the laboratory at the University of Colorado. The Core will have a large impact on the POI. as it provides tissue examination for in vivo models that compliment in vitro experiments. Comparative analysis of murine prostate cancers and clinical human prostate cancer specimens from different patient cohorts from two different laboratories will impact the field by clarifying signaling pathways in prostate cancer progression. The Core will have 3 Aims:
Aim 1. Histologic analysis of cell cultures, xenografts, mouse models, and clinical human prostate tissue specimens. Specimens will be processed as archival (formalin-fixed, paraffin-embedded) samples or as frozen sections. In addition, Drs. Frierson and Lucia will use their expertise as pathologists to examine the prostate glands of transgenic mice to determine histopathologic changes induced by the genetic modifications.
Aim 2. Immunohistochemical analysis and optimization of antibodies, specifically to determine their utility in formalin-fixed, paraffin-embedded material and. if necessary, in frozen sections. Drs. Frierson and Lucia will use the avidin-biotin immunoperoxidase technique and their immunohistochemical expertise to determine optimal reagent conditions and antibody staining in cell cultures, xenografts, mouse models, and clinical human prostate cancer specimens.
Aim 3. Selection, preparation, and procurement of cells for laser microdissection. Drs. Frierson and Lucia have the ability to perform laser microdissection of prostate glands from human prostate tissues, transgenic mice, and xenografts for molecular studies

Public Health Relevance

Core C will function as a tissue analysis laboratory to examine specimens from mouse models of prostate cancer. In addition, the laboratory will undertake translational studies in which human prostate cancers will be examined for important molecules which have been uncovered in in vitro and animal models of aggressive prostate cancer. Such analysis will lead to a better understanding ofthe molecular underpinnings of human prostate cancer.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
5P01CA104106-09
Application #
8744398
Study Section
Special Emphasis Panel (ZCA1)
Project Start
Project End
Budget Start
2014-09-01
Budget End
2015-08-31
Support Year
9
Fiscal Year
2014
Total Cost
Indirect Cost
Name
University of Virginia
Department
Type
DUNS #
City
Charlottesville
State
VA
Country
United States
Zip Code
22904
Kuscu, Canan; Kumar, Pankaj; Kiran, Manjari et al. (2018) tRNA fragments (tRFs) guide Ago to regulate gene expression post-transcriptionally in a Dicer-independent manner. RNA 24:1093-1105
Hao, Yi; Bjerke, Glen A; Pietrzak, Karolina et al. (2018) TGF? signaling limits lineage plasticity in prostate cancer. PLoS Genet 14:e1007409
Yang, Chun-Song; Melhuish, Tiffany A; Spencer, Adam et al. (2017) The protein kinase C super-family member PKN is regulated by mTOR and influences differentiation during prostate cancer progression. Prostate 77:1452-1467
Kumar, Pankaj; Kuscu, Canan; Dutta, Anindya (2016) Biogenesis and Function of Transfer RNA-Related Fragments (tRFs). Trends Biochem Sci 41:679-689
Agarwal, Neeraj; Dancik, Garrett M; Goodspeed, Andrew et al. (2016) GON4L Drives Cancer Growth through a YY1-Androgen Receptor-CD24 Axis. Cancer Res 76:5175-85
Reon, Brian J; Dutta, Anindya (2016) Biological Processes Discovered by High-Throughput Sequencing. Am J Pathol 186:722-32
Sakurai, Kouhei; Reon, Brian J; Anaya, Jordan et al. (2015) The lncRNA DRAIC/PCAT29 Locus Constitutes a Tumor-Suppressive Nexus. Mol Cancer Res 13:828-38
Dillon, Laura W; Kumar, Pankaj; Shibata, Yoshiyuki et al. (2015) Production of Extrachromosomal MicroDNAs Is Linked to Mismatch Repair Pathways and Transcriptional Activity. Cell Rep 11:1749-59
Kumar, Pankaj; Mudunuri, Suresh B; Anaya, Jordan et al. (2015) tRFdb: a database for transfer RNA fragments. Nucleic Acids Res 43:D141-5
Earl, Julie; Rico, Daniel; Carrillo-de-Santa-Pau, Enrique et al. (2015) The UBC-40 Urothelial Bladder Cancer cell line index: a genomic resource for functional studies. BMC Genomics 16:403

Showing the most recent 10 out of 62 publications