- CORE D The Animal Imaging and Cytometry Core will support all Projects of the Program by providing key services, including: generation, breeding, and maintenance of genetically modified mouse colonies, murine asthma models (ovalbumin allergen, dust mite, and intratracheal IL-25 and in vivo NOS electroportion), mouse airway hyper-reactivity measures, processing and histological analyses of mouse lung tissues, murine data repository, in vivo-molecular imaging by Positron Emission Tomography (PET) and Single Photon Emission Computerized Tomography (SPECT) for iNOS activity and adherent cell cytometric analyses for intracellular probes. All required instruments and expertise are found in the Core. The Core expedites results and reduces cost of Projects through the use of shared model systems. New model systems and functional imaging increase overall innovation of the Program.

Public Health Relevance

- CORE D The biology of asthma is a very complex. Centralization of expertise needed to conduct research that advances our understanding of asthma is therefore critical. We support projects investigating asthma pathobiology by providing models of asthma, generation and breeding of knockout animals relevant to asthma, and imaging and cytometric analysis of the lungs for pathways important in airway inflammation.

National Institute of Health (NIH)
National Heart, Lung, and Blood Institute (NHLBI)
Research Program Projects (P01)
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Heart, Lung, and Blood Initial Review Group (HLBP)
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Cleveland Clinic Lerner
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