- CORE D The Animal Imaging and Cytometry Core will support all Projects of the Program by providing key services, including: generation, breeding, and maintenance of genetically modified mouse colonies, murine asthma models (ovalbumin allergen, dust mite, and intratracheal IL-25 and in vivo NOS electroportion), mouse airway hyper-reactivity measures, processing and histological analyses of mouse lung tissues, murine data repository, in vivo-molecular imaging by Positron Emission Tomography (PET) and Single Photon Emission Computerized Tomography (SPECT) for iNOS activity and adherent cell cytometric analyses for intracellular probes. All required instruments and expertise are found in the Core. The Core expedites results and reduces cost of Projects through the use of shared model systems. New model systems and functional imaging increase overall innovation of the Program.
- CORE D The biology of asthma is a very complex. Centralization of expertise needed to conduct research that advances our understanding of asthma is therefore critical. We support projects investigating asthma pathobiology by providing models of asthma, generation and breeding of knockout animals relevant to asthma, and imaging and cytometric analysis of the lungs for pathways important in airway inflammation.
|Johnson, Collin G; Stober, Vandy P; Cyphert-Daly, Jaime M et al. (2018) High molecular weight hyaluronan ameliorates allergic inflammation and airway hyperresponsiveness in the mouse. Am J Physiol Lung Cell Mol Physiol :|
|Majors, Alana K; Chakravarti, Ritu; Ruple, Lisa M et al. (2018) Nitric oxide alters hyaluronan deposition by airway smooth muscle cells. PLoS One 13:e0200074|
|Sweeny, Elizabeth A; Singh, Anuradha Bharara; Chakravarti, Ritu et al. (2018) Glyceraldehyde-3-phosphate dehydrogenase is a chaperone that allocates labile heme in cells. J Biol Chem 293:14557-14568|
|Reichard, Andrew; Wanner, Nicholas; Stuehr, Eric et al. (2018) Quantification of airway fibrosis in asthma by flow cytometry. Cytometry A 93:952-958|
|Asosingh, Kewal; Weiss, Kelly; Queisser, Kimberly et al. (2018) Endothelial cells in the innate response to allergens and initiation of atopic asthma. J Clin Invest 128:3116-3128|
|Allawzi, Ayed M; Vang, Alexander; Clements, Richard T et al. (2018) Activation of Anoctamin-1 Limits Pulmonary Endothelial Cell Proliferation via p38-Mitogen-activated Protein Kinase-Dependent Apoptosis. Am J Respir Cell Mol Biol 58:658-667|
|Reichard, Andrew; Asosingh, Kewal (2018) The role of mitochondria in angiogenesis. Mol Biol Rep :|
|Ghosh, Arnab; Garee, Greer; Sweeny, Elizabeth A et al. (2018) Hsp90 chaperones hemoglobin maturation in erythroid and nonerythroid cells. Proc Natl Acad Sci U S A 115:E1117-E1126|
|Comhair, Suzy A A; Bochenek, Grazyna; Baicker-McKee, Sara et al. (2018) The utility of biomarkers in diagnosis of aspirin exacerbated respiratory disease. Respir Res 19:210|
|Dai, Yue; Haque, Mohammad Mahfuzul; Stuehr, Dennis J (2017) Restricting the conformational freedom of the neuronal nitric-oxide synthase flavoprotein domain reveals impact on electron transfer and catalysis. J Biol Chem 292:6753-6764|
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