Abstract

The Dana-Farber Cancer Institute has established a Molecular Biology Core Facility to help Institute laboratories more easily and cost effectively use molecular biology techniques and reagents such as oligonucleotides, synthetic peptides, microprotein sequencing, mass analysis, DNA sequencing, protein-ligand assays and electron microscopy of nucleic acids and nucleic acid-protein interactions. This application requests funds to partially cover costs not covered by a charge back system. The facility contains the following major equipment: Applied Biosystems 394 DNA/RNA Synthesizer, Applied Biosystems 380B DNA Synthesizer, Applied Biosystems 477 Protein Sequencer, 120A Analyzer, Applied Biosystems 430 Peptide Synthesizer, Hewlett Packard HP1090 Micropore Liquid Chromatograph, 2 Waters HPLC's Applied Biosystems 420/H-130 Amino Acid Analyzer, Finnigan Lasermat TOF Mass Analyzer, Pharmacia BIAcore biosensor, Applied Biosystems 373 DNA Sequencer, Zeiss EM 10A Electron Microscope. The facility is staffed by a facility director and 4 full time technicians who provide the different services as well as provided instruction to investigators who wish to use specific instruments themselves.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Center Core Grants (P30)
Project #
5P30AI028691-10
Application #
6099443
Study Section
Project Start
1998-08-01
Project End
1999-07-31
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
10
Fiscal Year
1998
Total Cost
Indirect Cost

  Institution

Name
Dana-Farber Cancer Institute
Department
Type
DUNS #
149617367
City
Boston
State
MA
Country
United States
Zip Code
02215

  Publications

Liu, Songtao; Fleysher, Roman; Fleysher, Lazar et al. (2010) Brain metabolites B1-corrected proton T1 mapping in the rhesus macaque at 3 T. Magn Reson Med 63:865-71
Liu, Songtao; Gonen, Oded; Fleysher, Roman et al. (2009) Metabolite proton T(2) mapping in the healthy rhesus macaque brain at 3 T. Magn Reson Med 62:1292-9
Ratai, Eva-Maria; Pilkenton, Sarah J; Greco, Jane B et al. (2009) In vivo proton magnetic resonance spectroscopy reveals region specific metabolic responses to SIV infection in the macaque brain. BMC Neurosci 10:63
Gonen, Oded; Liu, Songtao; Goelman, Gadi et al. (2008) Proton MR spectroscopic imaging of rhesus macaque brain in vivo at 7T. Magn Reson Med 59:692-9
Ratai, Eva-Maria; Hancu, Ileana; Blezek, Daniel J et al. (2008) Automatic repositioning of MRSI voxels in longitudinal studies: impact on reproducibility of metabolite concentration measurements. J Magn Reson Imaging 27:1188-93
Liu, Songtao; Gonen, Oded; Fleysher, Lazar et al. (2008) Regional metabolite T2 in the healthy rhesus macaque brain at 7T. Magn Reson Med 59:1165-9
Li, Xing; Gold, Bert; O'hUigin, Colm et al. (2007) Unique features of TRIM5alpha among closely related human TRIM family members. Virology 360:419-33
Javanbakht, Hassan; An, Ping; Gold, Bert et al. (2006) Effects of human TRIM5alpha polymorphisms on antiretroviral function and susceptibility to human immunodeficiency virus infection. Virology 354:15-27
Diaz-Griffero, Felipe; Li, Xing; Javanbakht, Hassan et al. (2006) Rapid turnover and polyubiquitylation of the retroviral restriction factor TRIM5. Virology 349:300-15
Gonzalez, R Gilberto; Greco, Jane B; He, Julian et al. (2006) New insights into the neuroimmunity of SIV infection by magnetic resonance spectroscopy. J Neuroimmune Pharmacol 1:152-9

Showing the most recent 10 out of 205 publications