Genetically engineered mice and rats are widely used as models for defining the molecular mechanisms underlying cancer etiology and for evaluating new strategies for cancer treatment and prevention. The Genome Editing and Animal Models Shared Resource (GEAM), formerly the Transgenic and Mutant Animal Facility, has served as a shared resource for the University of Wisconsin Carbone Cancer Center (UWCCC) for over two decades and is one of the elite facilities within the United States in offering a comprehensive array of services related to generation and preservation of genome-edited animal models. The primary mission of the GEAM is to make state-of-the-art genome editing technologies accessible to UWCCC members.
Specific Aim 1 is to provide the expertise and infrastructure required to generate novel and relevant genome-edited or transgenic animal models and genome-edited cell models for use in cancer research. GEAM staff are capable of serving UWCCC members in all aspects of experiment planning and execution, including design of efficient and specific approaches to achieve the desired loss, gain, or alteration of gene function using CRISPR/Cas9 or other genome editing and transgene-based approaches; design, production and use of the required genome editing reagents or transgene vectors; identification of animals that carry the desired genome edit or transgene; and minimization of off target edits. Our skills in reproductive biology, embryo manipulation, and animal husbandry enable us to edit the genomes of inbred mouse and rat strains that exhibit low reproductive capacity.
Specific Aim 2 is to provide state-of-the-art services that allow valuable animal models to be banked and recovered as needed to preserve these animal models or reduce the costs associated with maintaining live breeding stock for novel models that are not actively being studied. GEAM staff are highly experienced and capable of cryopreserving mouse and rat embryos or sperm and recovering mouse and rat models through embryo transfer or in vitro fertilization. In addition, we are capable of rederiving mouse and rat strains to eliminate pathogens that may compromise research or prevent animal models from being imported into our vivaria or shared between investigators working at different institutions. Since its inception, GEAM has generated hundreds of transgenic, knockout, and genome-edited mouse and rat models for UWCCC members. Sixty-one unique UWCCC members have been served during the current CCSG funding cycle, an increase of 30% compared to the previous grant cycle. Support from the CCSG allows our services to be provided to UWCCC members at costs far below those of commercial vendors or similar cores at other research universities. Convenient access to our first rate, cost-effective services enhances the ability of UWCCC investigators to conduct innovative research that advances the UWCCC strategic mission.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA014520-46
Application #
9923030
Study Section
Subcommittee I - Transistion to Independence (NCI)
Project Start
Project End
Budget Start
2020-04-01
Budget End
2021-03-31
Support Year
46
Fiscal Year
2020
Total Cost
Indirect Cost
Name
University of Wisconsin Madison
Department
Type
DUNS #
161202122
City
Madison
State
WI
Country
United States
Zip Code
53715
Wu, Yirong; Fan, Jun; Peissig, Peggy et al. (2018) Quantifying predictive capability of electronic health records for the most harmful breast cancer. Proc SPIE Int Soc Opt Eng 10577:
Fu, Anqi; Oberholtzer, Sydney M; Bagheri-Fam, Stefan et al. (2018) Dynamic expression patterns of Irx3 and Irx5 during germline nest breakdown and primordial follicle formation promote follicle survival in mouse ovaries. PLoS Genet 14:e1007488
Ni, Dalong; Jiang, Dawei; Kutyreff, Christopher J et al. (2018) Molybdenum-based nanoclusters act as antioxidants and ameliorate acute kidney injury in mice. Nat Commun 9:5421
Huynh, Mailee; Pak, Chorom; Markovina, Stephanie et al. (2018) Hyaluronan and proteoglycan link protein 1 (HAPLN1) activates bortezomib-resistant NF-?B activity and increases drug resistance in multiple myeloma. J Biol Chem 293:2452-2465
Farrell, Emily; Armstrong, Annie E; Grimes, Adrian C et al. (2018) Transcriptome Analysis of Cardiac Hypertrophic Growth in MYBPC3-Null Mice Suggests Early Responders in Hypertrophic Remodeling. Front Physiol 9:1442
Net, Jose M; Whitman, Gary J; Morris, Elizabteh et al. (2018) Relationships Between Human-Extracted MRI Tumor Phenotypes of Breast Cancer and Clinical Prognostic Indicators Including Receptor Status and Molecular Subtype. Curr Probl Diagn Radiol :
Jiang, Dawei; Ge, Zhilei; Im, Hyung-Jun et al. (2018) DNA origami nanostructures can exhibit preferential renal uptake and alleviate acute kidney injury. Nat Biomed Eng 2:865-877
Seok, Seung-Hyeon; Ma, Zhi-Xiong; Feltenberger, John B et al. (2018) Trace derivatives of kynurenine potently activate the aryl hydrocarbon receptor (AHR). J Biol Chem 293:1994-2005
Chapelin, Fanny; Capitini, Christian M; Ahrens, Eric T (2018) Fluorine-19 MRI for detection and quantification of immune cell therapy for cancer. J Immunother Cancer 6:105
Ni, Dalong; Ferreira, Carolina A; Barnhart, Todd E et al. (2018) Magnetic Targeting of Nanotheranostics Enhances Cerenkov Radiation-Induced Photodynamic Therapy. J Am Chem Soc 140:14971-14979

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