Genetically engineered mice and rats are widely used as models for defining the molecular mechanisms underlying cancer etiology and for evaluating new strategies for cancer treatment and prevention. The Genome Editing and Animal Models Shared Resource (GEAM), formerly the Transgenic and Mutant Animal Facility, has served as a shared resource for the University of Wisconsin Carbone Cancer Center (UWCCC) for over two decades and is one of the elite facilities within the United States in offering a comprehensive array of services related to generation and preservation of genome-edited animal models. The primary mission of the GEAM is to make state-of-the-art genome editing technologies accessible to UWCCC members.
Specific Aim 1 is to provide the expertise and infrastructure required to generate novel and relevant genome-edited or transgenic animal models and genome-edited cell models for use in cancer research. GEAM staff are capable of serving UWCCC members in all aspects of experiment planning and execution, including design of efficient and specific approaches to achieve the desired loss, gain, or alteration of gene function using CRISPR/Cas9 or other genome editing and transgene-based approaches; design, production and use of the required genome editing reagents or transgene vectors; identification of animals that carry the desired genome edit or transgene; and minimization of off target edits. Our skills in reproductive biology, embryo manipulation, and animal husbandry enable us to edit the genomes of inbred mouse and rat strains that exhibit low reproductive capacity.
Specific Aim 2 is to provide state-of-the-art services that allow valuable animal models to be banked and recovered as needed to preserve these animal models or reduce the costs associated with maintaining live breeding stock for novel models that are not actively being studied. GEAM staff are highly experienced and capable of cryopreserving mouse and rat embryos or sperm and recovering mouse and rat models through embryo transfer or in vitro fertilization. In addition, we are capable of rederiving mouse and rat strains to eliminate pathogens that may compromise research or prevent animal models from being imported into our vivaria or shared between investigators working at different institutions. Since its inception, GEAM has generated hundreds of transgenic, knockout, and genome-edited mouse and rat models for UWCCC members. Sixty-one unique UWCCC members have been served during the current CCSG funding cycle, an increase of 30% compared to the previous grant cycle. Support from the CCSG allows our services to be provided to UWCCC members at costs far below those of commercial vendors or similar cores at other research universities. Convenient access to our first rate, cost-effective services enhances the ability of UWCCC investigators to conduct innovative research that advances the UWCCC strategic mission.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA014520-46
Application #
9923030
Study Section
Subcommittee I - Transistion to Independence (NCI)
Project Start
Project End
Budget Start
2020-04-01
Budget End
2021-03-31
Support Year
46
Fiscal Year
2020
Total Cost
Indirect Cost
Name
University of Wisconsin Madison
Department
Type
DUNS #
161202122
City
Madison
State
WI
Country
United States
Zip Code
53715
Rutter, Carolyn M; Kim, Jane J; Meester, Reinier G S et al. (2018) Effect of Time to Diagnostic Testing for Breast, Cervical, and Colorectal Cancer Screening Abnormalities on Screening Efficacy: A Modeling Study. Cancer Epidemiol Biomarkers Prev 27:158-164
Jadvar, Hossein; Chen, Xiaoyuan; Cai, Weibo et al. (2018) Radiotheranostics in Cancer Diagnosis and Management. Radiology 286:388-400
Denu, Ryan A; Shabbir, Maria; Nihal, Minakshi et al. (2018) Centriole Overduplication is the Predominant Mechanism Leading to Centrosome Amplification in Melanoma. Mol Cancer Res 16:517-527
England, Christopher G; Jiang, Dawei; Ehlerding, Emily B et al. (2018) 89Zr-labeled nivolumab for imaging of T-cell infiltration in a humanized murine model of lung cancer. Eur J Nucl Med Mol Imaging 45:110-120
Ong, Irene M; Gonzalez, Jose G; McIlwain, Sean J et al. (2018) Gut microbiome populations are associated with structure-specific changes in white matter architecture. Transl Psychiatry 8:6
Trentham-Dietz, Amy; Ergun, Mehmet Ali; Alagoz, Oguzhan et al. (2018) Comparative effectiveness of incorporating a hypothetical DCIS prognostic marker into breast cancer screening. Breast Cancer Res Treat 168:229-239
Weiss, Jennifer M; Pandhi, Nancy; Kraft, Sally et al. (2018) Primary care colorectal cancer screening correlates with breast cancer screening: implications for colorectal cancer screening improvement interventions. Clin Transl Gastroenterol 9:148
Bruce, Jordan G; Tucholka, Jennifer L; Steffens, Nicole M et al. (2018) Feasibility of Providing Web-Based Information to Breast Cancer Patients Prior to a Surgical Consult. J Cancer Educ 33:1069-1074
Huynh, Mailee; Pak, Chorom; Markovina, Stephanie et al. (2018) Hyaluronan and proteoglycan link protein 1 (HAPLN1) activates bortezomib-resistant NF-?B activity and increases drug resistance in multiple myeloma. J Biol Chem 293:2452-2465
Wu, Yirong; Fan, Jun; Peissig, Peggy et al. (2018) Quantifying predictive capability of electronic health records for the most harmful breast cancer. Proc SPIE Int Soc Opt Eng 10577:

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