The Laboratory for Cell Analysis (LCA) is a core facility that provides cytometric support to the UCSF Helen Diller Family Comprehensive Cancer Center (Center). The LCA provides: ? access to flow and image cytometers, including cell sorters, confocal microscopes, digital pathology and laser microdissection systems, as well as technical support needed to make use of these instruments. ? education and hands-on training for students, staff, and faculty in cytometry technology and applications. ? expertise for the development of new cytometric assays, analytic software, and new instrumentation;as well as the interpretation and presentation of results. Instruments currently operated by the LCA include: four Becton-Dickinson (BD) FACS Aria systems with high-speed cell sorting, with minimally four laser excitation, ten detectors and single-cell cloning;two BD LSRII flow cytometers;a FACS Canto II;three bench-top flow cytometers;one Zeiss 510 Confocal Laser Scanning Microscope, with multi-photon lasers, spectral emission detection and live cell scanning capability;one Zeiss Spinning Disc Confocal system with TIRF;four digital video fluorescent microscopes with high resolution CCD cameras;a Nuance Spectral imaging system;a Zeiss PALM Laser Micro Dissection microscope consisting of a robotic cutting system with non-contact sample collection. A variety of computer software packages and platforms are available to collect and analyze acquired data along with links to offline databases and statistical processing routines for more advance processing. In addition, the staff of the LCA offers their expertise in the use of this technology as it applies to instrument support and biological applications for CC members. The LCA website is http://cancer.ucsf.edu/lca/index.php.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
2P30CA082103-14
Application #
8388372
Study Section
Subcommittee G - Education (NCI)
Project Start
1999-08-05
Project End
2017-05-31
Budget Start
2012-09-19
Budget End
2013-05-31
Support Year
14
Fiscal Year
2012
Total Cost
$328,345
Indirect Cost
$118,652
Name
University of California San Francisco
Department
Type
DUNS #
094878337
City
San Francisco
State
CA
Country
United States
Zip Code
94143
Dvorak, Christopher C; Satwani, Prakash; Stieglitz, Elliot et al. (2018) Disease burden and conditioning regimens in ASCT1221, a randomized phase II trial in children with juvenile myelomonocytic leukemia: A Children's Oncology Group study. Pediatr Blood Cancer 65:e27034
Fan, Qi Wen; Nicolaides, Theodore P; Weiss, William A (2018) Inhibiting 4EBP1 in Glioblastoma. Clin Cancer Res 24:14-21
Sannino, Sara; Guerriero, Christopher J; Sabnis, Amit J et al. (2018) Compensatory increases of select proteostasis networks after Hsp70 inhibition in cancer cells. J Cell Sci 131:
Lam, Christine; Ferguson, Ian D; Mariano, Margarette C et al. (2018) Repurposing tofacitinib as an anti-myeloma therapeutic to reverse growth-promoting effects of the bone marrow microenvironment. Haematologica 103:1218-1228
Truillet, Charles; Parker, Matthew F L; Huynh, Loc T et al. (2018) Measuring glucocorticoid receptor expression in vivo with PET. Oncotarget 9:20399-20408
Phillips, Kathryn A; Trosman, Julia R; Deverka, Patricia A et al. (2018) Insurance coverage for genomic tests. Science 360:278-279
Phillips, Kathryn A (2018) Evolving Payer Coverage Policies on Genomic Sequencing Tests: Beginning of the End or End of the Beginning? JAMA 319:2379-2380
Puri, Sapna; Roy, Nilotpal; Russ, Holger A et al. (2018) Replication confers ? cell immaturity. Nat Commun 9:485
An, Zhenyi; Aksoy, Ozlem; Zheng, Tina et al. (2018) Epidermal growth factor receptor and EGFRvIII in glioblastoma: signaling pathways and targeted therapies. Oncogene 37:1561-1575
Behr, Spencer C; Villanueva-Meyer, Javier E; Li, Yan et al. (2018) Targeting iron metabolism in high-grade glioma with 68Ga-citrate PET/MR. JCI Insight 3:

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