Central to the understanding of metal protein interactions has been the question of whether metals enforce specific quarternary structure in proteins or whether proteins impart a specific coordination environment on the metal. Several amphiphilic, a-helical peptides have been designed de novo in order to permit a direct experimental determination of the factors which define metal protein interactions. By placing cysteine residues into the interior of the bundle to generate metal binding sites, we can test directly the stability of a particular bundle and the role of metal-ion coordination geometry in altering this stability. We have chosen a series of thiophilic metals that have coordination preferences including 2 coordinate (linear), 3 coordinate (trigonal) and 4 coordinate (tetrahedral) to deduce the thermodynamic stabilities associated with metal binding in these different environments. The metals to be studied are Zn(II), Hg(II), Cd(II), Cu(I), Ag(I) and Co(II). With the exception of the Co(II), which serves as a control, there is no technique other than EXAFS that can provide unambigous characterization of the local metal-site structure.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
3P41RR001209-23S1
Application #
6658651
Study Section
Project Start
2002-03-01
Project End
2003-02-28
Budget Start
Budget End
Support Year
23
Fiscal Year
2002
Total Cost
$143,176
Indirect Cost
Name
Stanford University
Department
Type
DUNS #
800771545
City
Stanford
State
CA
Country
United States
Zip Code
94305
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