It is proposed to operate a Biotechnology Research Center using high-energy x-rays produced by the National Synchrotron Light Source (NSLS) for trace element analysis in a number of biomedical fields. The uniquely high x-ray intensity from the NSLS will be used as the basis of an X-Ray Microprobe Facility which will be able to provide a highly polarized photon flux of about 10 to the 15 photons/square centimeters/sec in a spot of size 2 mm x 2mm. With use of high resolution electron and photon spectrometers multielement detection for essentially all elements in the periodic table will be possible with sensitivities close to one part per billion by weight in times of 5 minutes or less for each sample. A two-dimensional demagnification system will be used to provide focussed spots of 20 Mum or less with similar detection sensitivities. The properties of this system will be investigated to determine the ultimate sensitivities and spatial resolutions that can be obtained. Round-robin studies will be carried out to compare this technique with other analytical methods and to demonstrate its unique features. Studies on selected biomedical topics will be carried out on a collaborative basis.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR001838-02
Application #
3104016
Study Section
(SSS)
Project Start
1984-06-01
Project End
1987-05-31
Budget Start
1985-06-01
Budget End
1986-05-31
Support Year
2
Fiscal Year
1985
Total Cost
Indirect Cost
Name
Associated University-Brookhaven National Lab
Department
Type
DUNS #
City
Upton
State
NY
Country
United States
Zip Code
11973
Jones, K W; Bockman, R S; Bronner, F (1992) Microdistribution of lead in bone: a new approach. Neurotoxicology 13:835-41
Vittur, F; Tuniz, C; Paoletti, S et al. (1992) Elemental analysis of growth plate cartilage by synchrotron-radiation-induced X-ray emission (SRIXE). Biochem Biophys Res Commun 188:1010-7
Meshinchi, S; Stancato, L F; Gordon, B M et al. (1991) Purification of the endogenous glucocorticoid receptor stabilizing factor. Biochemistry 30:8617-22
Gordon, B M; Jones, K W; Hanson, A L et al. (1990) An X-ray microprobe facility using synchrotron radiation. Biol Trace Elem Res 26-27:133-41
Pounds, J G (1990) The role of cell calcium in current approaches to toxicology. Environ Health Perspect 84:7-15
Jones, K W; Schidlovsky, G; Burger, D E et al. (1990) Distribution of lead in human bone: III. Synchrotron x-ray microscope measurements. Basic Life Sci 55:281-6
Schidlovsky, G; Jones, K W; Burger, D E et al. (1990) Distribution of lead in human bone: II. Proton microprobe measurements. Basic Life Sci 55:275-80
Batuman, V; Wedeen, R P; Bogden, J D et al. (1989) Reducing bone lead content by chelation treatment in chronic lead poisoning: an in vivo X-ray fluorescence and bone biopsy study. Environ Res 48:70-5
Giauque, R D; Thompson, A C; Underwood, J H et al. (1988) Measurement of femtogram quantities of trace elements using an X-ray microprobe. Anal Chem 60:855-8
Jones, K W; Gordon, B M; Hanson, A L et al. (1988) X-ray fluorescence with synchrotron radiation. Ultramicroscopy 24:313-28

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