A laboratory animal model for the human lymphatic-dwelling filaria, Wuchereria bancrofti has been lacking. To date, infections have been established in 6 of 11 patas monkeys inoculated with infective stage larvae of the parasite. The first 10 patas were inoculated with larvae from mosquitoes that fed on infected human blood. The most recent was an animal inoculated with larvae collected from Culex quinquefasciatus (Haiti strain) mosquitoes that were infected by blood feeding directly on another patas with circulating microfilariae.
The aim was to passage the parasite in monkeys in the hope that it would become better adapted to the monkey host. The monkey developed an uncommonly high microfilaremia (95 mf per 20 fl), but despite this, the infectivity rate of mosquitoes that blood fed on it was low. Whether this was due to the development of resistance in the Haiti mosquito strain or diminished infectivity of the microfilariae due to passage was investigated. The Covington strain of Cx. quinquefasciatus was established as a control. A portion of both mosquito strains was blood fed directly on the monkey, and the remainder were fed on infected blood from a Haitian patient (cryopreserved blood). Only a few larvae developed in the Haiti strain of the mosquito after feeding on both the monkey and the human blood. For the Covington mosquito strain, only a few larvae developed after feeding on the monkey blood, but numerous larvae were recovered from mosquitoes that fed on the human blood. These results indicate that the Haiti strain of Cx. quinquefasciatus, which now is 178 passages from the field, has indeed become refractory to infection, but more importantly it shows that passage of the parasite in a monkey has considerably diminished the ability of microfilariae to infect mosquitoes.

National Institute of Health (NIH)
National Center for Research Resources (NCRR)
Primate Research Center Grants (P51)
Project #
Application #
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Mahalingam, Ravi; Kaufer, Benedikt B; Ouwendijk, Werner J D et al. (2018) Attenuation of Simian Varicella Virus Infection by Enhanced Green Fluorescent Protein in Rhesus Macaques. J Virol 92:
Kumar, Vinay; Mansfield, Joshua; Fan, Rong et al. (2018) miR-130a and miR-212 Disrupt the Intestinal Epithelial Barrier through Modulation of PPAR? and Occludin Expression in Chronic Simian Immunodeficiency Virus-Infected Rhesus Macaques. J Immunol 200:2677-2689
Parthasarathy, Geetha; Philipp, Mario T (2018) Intracellular TLR7 is activated in human oligodendrocytes in response to Borrelia burgdorferi exposure. Neurosci Lett 671:38-42
McNamara, Ryan P; Costantini, Lindsey M; Myers, T Alix et al. (2018) Nef Secretion into Extracellular Vesicles or Exosomes Is Conserved across Human and Simian Immunodeficiency Viruses. MBio 9:
Calenda, Giulia; Villegas, Guillermo; Barnable, Patrick et al. (2017) MZC Gel Inhibits SHIV-RT and HSV-2 in Macaque Vaginal Mucosa and SHIV-RT in Rectal Mucosa. J Acquir Immune Defic Syndr 74:e67-e74
Datta, Dibyadyuti; Bansal, Geetha P; Grasperge, Brooke et al. (2017) Comparative functional potency of DNA vaccines encoding Plasmodium falciparum transmission blocking target antigens Pfs48/45 and Pfs25 administered alone or in combination by in vivo electroporation in rhesus macaques. Vaccine 35:7049-7056
Yi, Fei; Guo, Jia; Dabbagh, Deemah et al. (2017) Discovery of Novel Small-Molecule Inhibitors of LIM Domain Kinase for Inhibiting HIV-1. J Virol 91:
Jorgensen, Matthew J; Lambert, Kelsey R; Breaux, Sarah D et al. (2017) Pair housing of Vervets/African Green Monkeys for biomedical research. Am J Primatol 79:1-10
Ramesh, Geeta; Martinez, Alejandra N; Martin, Dale S et al. (2017) Effects of dexamethasone and meloxicam on Borrelia burgdorferi-induced inflammation in glial and neuronal cells of the central nervous system. J Neuroinflammation 14:28
Parthasarathy, Geetha; Philipp, Mario T (2017) Receptor tyrosine kinases play a significant role in human oligodendrocyte inflammation and cell death associated with the Lyme disease bacterium Borrelia burgdorferi. J Neuroinflammation 14:110

Showing the most recent 10 out of 352 publications