This application's long-term objectives are investigation of neuroendocrine changes responsible for age-related loss of reproductive cycles.
The specific aims of this application are to use the technique of high performance liquid chromatography to determine age-related changes in the activities of enzymes involved in the synthesis and degradation of catecholamines and serotonin in discrete areas of the hypothalamus. Age-related changes in the contents and release of catecholamines and serotonin and gonadotropin releasing hormone from discrete areas of the hypothalamus will also be determined. The technique of push-pull perfusion will be used to determine the release of neurotransmitters and gonadotropin releasing hormone from hypothalamic areas. The data on the activities of the enzymes and on the content and release of neurotransmitters will be collected on a long-term basis during various patterns of acyclicity and correlated with serum levels of luteinizing hormone, prolactin, estradiol and progesterone and with vaginal cytology. Changes in these parameters will also be determined after acyclic animals have been treated with -dopa and after normal cyclic animals have been on chronic treatment with estradiol. The data collected in this project will explain in detail the age- related changes in the activities of the enzymes involved in the synthesis and degradation of catecholamines and serotonin and in the hypothalamic content and release of these neurotransmitters. These data will be new and will add to our understanding of the primary causes of loss of reproductive cyclicity in aging.

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Research Project (R01)
Project #
1R01AG005980-01A2
Application #
3116750
Study Section
Reproductive Biology Study Section (REB)
Project Start
1987-08-01
Project End
1990-07-31
Budget Start
1987-08-01
Budget End
1988-07-31
Support Year
1
Fiscal Year
1987
Total Cost
Indirect Cost
Name
Kansas State University
Department
Type
Schools of Veterinary Medicine
DUNS #
City
Manhattan
State
KS
Country
United States
Zip Code
66506
Clark, Kimberly A; MohanKumar, Sheba M J; Kasturi, Badrinarayanan S et al. (2006) Effects of central and systemic administration of leptin on neurotransmitter concentrations in specific areas of the hypothalamus. Am J Physiol Regul Integr Comp Physiol 290:R306-12
Francis, Joseph; MohanKumar, Sheba M J; MohanKumar, P S (2004) Leptin inhibits norepinephrine efflux from the hypothalamus in vitro: role of gamma aminobutyric acid. Brain Res 1021:286-91
MohanKumar, Sheba M J; MohanKumar, P S (2004) Aging alters norepinephrine release in the medial preoptic area in response to steroid priming in ovariectomized rats. Brain Res 1023:24-30
MohanKumar, Sheba M J; Smith, Courtney L; MohanKumar, P S (2003) Central adaptation to chronic administration of interleukin-1beta (IL-1beta) in rats. Brain Res Bull 62:71-6
Barber, Matthew; Kasturi, Badrinarayanan S; Austin, Maureen E et al. (2003) Diabetes-induced neuroendocrine changes in rats: role of brain monoamines, insulin and leptin. Brain Res 964:128-35
Austin, Maureen E; Kasturi, Badrinarayanan S; Barber, Matthew et al. (2003) Neuroendocrine effects of perfluorooctane sulfonate in rats. Environ Health Perspect 111:1485-9
MohanKumar, Sheba M J; MohanKumar, P S (2002) Effects of interleukin-1 beta on the steroid-induced luteinizing hormone surge: role of norepinephrine in the medial preoptic area. Brain Res Bull 58:405-9
MohanKumar, P S; MohanKumar, S M; Quadri, S K (2001) Deprenyl stimulates the efflux of monoamines from the rat hypothalamus in vitro. Brain Res Bull 54:675-80
Francis, J; MohanKumar, P S; MohanKumar, S M (2001) Lipopolysaccharide stimulates norepinephrine efflux from the rat hypothalamus in vitro: blockade by soluble IL-1 receptor. Neurosci Lett 308:71-4
Weiss, M L; Dobbs, M E; MohanKumar, P S et al. (2001) The estrous cycle affects pseudorabies virus (PRV) infection of the CNS. Brain Res 893:215-26

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