The long term objective of tis proposal is to learn the etiology and the pathogenesis of Alzheimer disease/senile dementia of the Alzheimer type (AD/SDAT) which constitutes one of the major public health problems in modern society. In the United States alone presently over three million senior citizens are affected and these numbers will keep increasing at a frightening rate unless the disease is understood and prevented. Several lines of evidence suggest that the state of phosphorylation/ dephosphorylation of neuronal proteins including the microtubule associated proteins tau might be affected in AD/SDAT and that the Alzheimer paired helical filaments (PHF) might contain abnormally phosphorylated tau.
The specific aims of this project are 1) a thorough investigation of the ability of Alzheimer brain tau and PHF, with or without prior in vitro dephosphorylation, to stimulate in vitro assembly of microtubules from bovine tubulin-determined by turbidimetric measurements, negative stain electron microscopy and SDS-PAGE; 2) identification of the protein kinase/s responsible for the phosphorylation of tau and PHF in AD/SDAT brain and measurements of this kinase activity in AD/SDAT and in age-matched unaffected brains-- determined by autoradiography and Western blots of SDS-PAGE; and 3) study the effect of phosphorylation on the self assembly of tau into filaments -- determined by negative stain electron microscopy, turbidimetric measurements and SDS-PAGE. The studies will test whether the abnormal phosphorylation of tau might be the cause of the microtubule assembly defect in AD/SDAT brain and whether dephosphorylation of tau/PHF can reverse this defect in vitro. Identification of the protein kinase/s responsible for the phosphorylation of tau in AD/SDAT brain and determination of the conditions for the self assembly of tau into filaments and their depolymerization might be critical to devise a rational approach in correcting this defect. These studies will help eluciate how alterations of the normal cytoskeleton might contribute to neurofibrillary pathology and functional deficits in AD/SDAT brain.

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Research Project (R01)
Project #
1R01AG008076-01A3
Application #
3119465
Study Section
Neurology A Study Section (NEUA)
Project Start
1991-02-01
Project End
1995-01-31
Budget Start
1991-02-01
Budget End
1992-01-31
Support Year
1
Fiscal Year
1991
Total Cost
Indirect Cost
Name
Institute for Basic Research in Dev Disabil
Department
Type
DUNS #
167205090
City
Staten Island
State
NY
Country
United States
Zip Code
10314
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