The high affinity, siderophore mediated iron assimilation systems of prokaryotic and eukaryotic microorganisms will be investigated by application of the standard techniques of molecular genetics. The biosynthesis and transport components of the cloned, plasmid-derived, aerobactin system of hospital isolates of Escherichia coli will be defined. Definitive proof for the pathway from lysine to N omega-hydroxylysine to aerobactin will be sought and the enzymes characterized. The nature of the transcriptional regulation of this system by iron will be studied to determine if the message accumulated at low iron results from iron-repressor binding to the promoter-operator or, alternatively, from altered rates of turnover of the mRNA. Preliminary experiments will be aimed at cloning all or part of the rhodotorulic acid siderophore system of the basidiomycetous yeast Rhodotorula pilimanae.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI004156-27
Application #
3124177
Study Section
Microbial Physiology and Genetics Subcommittee 2 (MBC)
Project Start
1976-05-01
Project End
1990-04-30
Budget Start
1988-05-01
Budget End
1989-04-30
Support Year
27
Fiscal Year
1988
Total Cost
Indirect Cost
Name
University of California Berkeley
Department
Type
Schools of Arts and Sciences
DUNS #
094878337
City
Berkeley
State
CA
Country
United States
Zip Code
94704
Jasmer, Douglas P; Goverse, Aska; Smant, Geert (2003) Parasitic nematode interactions with mammals and plants. Annu Rev Phytopathol 41:245-70
Coy, M (1995) The interaction of the ferric uptake regulation protein with DNA. Biochem Biophys Res Commun 212:784-92
Persmark, M; Neilands, J B (1992) Iron(III) complexes of chrysobactin, the siderophore of Erwinia chrysanthemi. Biometals 5:29-36
Persmark, M; Expert, D; Neilands, J B (1992) Ferric iron uptake in Erwinia chrysanthemi mediated by chrysobactin and related catechol-type compounds. J Bacteriol 174:4783-9
Coy, M; Neilands, J B (1991) Structural dynamics and functional domains of the fur protein. Biochemistry 30:8201-10
Neilands, J B (1990) Molecular aspects of regulation of high affinity iron absorption in microorganisms. Adv Inorg Biochem 8:63-90
Gill Jr, P R; Neilands, J B (1989) Cloning a genomic region required for a high-affinity iron-uptake system in Rhizobium meliloti 1021. Mol Microbiol 3:1183-9
Persmark, M; Expert, D; Neilands, J B (1989) Isolation, characterization, and synthesis of chrysobactin, a compound with siderophore activity from Erwinia chrysanthemi. J Biol Chem 264:3187-93
Wee, S; Neilands, J B; Bittner, M L et al. (1988) Expression, isolation and properties of Fur (ferric uptake regulation) protein of Escherichia coli K 12. Biol Met 1:62-8
Schwyn, B; Neilands, J B (1987) Universal chemical assay for the detection and determination of siderophores. Anal Biochem 160:47-56

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