The long-term objective of this research is to provide information on the mode of iron absorption in microbial species, with emphasis on the molecular mechanism of regulation. Insufficient iron leads to anemia in animal subject and to impaired crop yields in plants. Excess iron promotes infection and results in generation of tissue damaging oxygen radicals. A combination of biochemical and molecular biological methods will be used for the study of iron absorption in two enteric bacterial species, Escherichia coli and Salmonella typhimurium, and in the single-celled basidiomycetous fungus, Rhodotorula pilimanae.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI004156-30
Application #
3124179
Study Section
Microbial Physiology and Genetics Subcommittee 2 (MBC)
Project Start
1976-05-01
Project End
1994-04-30
Budget Start
1991-05-01
Budget End
1994-04-30
Support Year
30
Fiscal Year
1991
Total Cost
Indirect Cost
Name
University of California Berkeley
Department
Type
Schools of Arts and Sciences
DUNS #
094878337
City
Berkeley
State
CA
Country
United States
Zip Code
94704
Jasmer, Douglas P; Goverse, Aska; Smant, Geert (2003) Parasitic nematode interactions with mammals and plants. Annu Rev Phytopathol 41:245-70
Coy, M (1995) The interaction of the ferric uptake regulation protein with DNA. Biochem Biophys Res Commun 212:784-92
Persmark, M; Neilands, J B (1992) Iron(III) complexes of chrysobactin, the siderophore of Erwinia chrysanthemi. Biometals 5:29-36
Persmark, M; Expert, D; Neilands, J B (1992) Ferric iron uptake in Erwinia chrysanthemi mediated by chrysobactin and related catechol-type compounds. J Bacteriol 174:4783-9
Coy, M; Neilands, J B (1991) Structural dynamics and functional domains of the fur protein. Biochemistry 30:8201-10
Neilands, J B (1990) Molecular aspects of regulation of high affinity iron absorption in microorganisms. Adv Inorg Biochem 8:63-90
Gill Jr, P R; Neilands, J B (1989) Cloning a genomic region required for a high-affinity iron-uptake system in Rhizobium meliloti 1021. Mol Microbiol 3:1183-9
Persmark, M; Expert, D; Neilands, J B (1989) Isolation, characterization, and synthesis of chrysobactin, a compound with siderophore activity from Erwinia chrysanthemi. J Biol Chem 264:3187-93
Wee, S; Neilands, J B; Bittner, M L et al. (1988) Expression, isolation and properties of Fur (ferric uptake regulation) protein of Escherichia coli K 12. Biol Met 1:62-8
Schwyn, B; Neilands, J B (1987) Universal chemical assay for the detection and determination of siderophores. Anal Biochem 160:47-56

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