Abstract

The DNA base sequence of the pI promoter of the lambda-related phage 21 will be determined. We have already sequenced pI from phage 434. Phage 21 is of interest because, unlike 434, it has a different insertion site than lambda. However, its pI promoter responds to the cII protein. Mutations in the pI promoter of lambda will be identified and sequenced. The structural gene for biotin sulfoxide reductase (bisC) will be cloned on a multi-copy plasmid. This should provide us with sufficient bisC protein to obtain a pure product, as well as facilitating genetic analysis. Insertions of Mu Ap 1ac in bisC will be selected so that the regulation, if any, of bisC can be examined. We have found that this enzyme uses the same molybdenum cofactor (product of genes ch1A, ch1B, ch1E, ch1G) as nitrate reductase. The relation of those genes to bisC regulation will be tested. The birA gene, structural gene for the bifunctional protein holoenzyme-synthetase biotin-repressor, already cloned as a 2.1 kb segment, will be sequenced in wild type and mutant form.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI008573-18
Application #
3124420
Study Section
Microbial Physiology and Genetics Subcommittee 2 (MBC)
Project Start
1978-06-01
Project End
1988-05-31
Budget Start
1985-06-01
Budget End
1986-05-31
Support Year
18
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Stanford University
Department
Type
Schools of Arts and Sciences
DUNS #
800771545
City
Stanford
State
CA
Country
United States
Zip Code
94305

  Publications

Campbell, A (1994) Comparative molecular biology of lambdoid phages. Annu Rev Microbiol 48:193-222
Campbell, A M (1993) Thirty years ago in Genetics: prophage insertion into bacterial chromosomes. Genetics 133:433-7
Campbell, A M (1993) Genome organization in prokaryotes. Curr Opin Genet Dev 3:837-44
Campbell, A M (1993) Co-chairman's remarks: genetic recombination in the molecular era. Gene 135:147-51
Karlin, S; Burge, C; Campbell, A M (1992) Statistical analyses of counts and distributions of restriction sites in DNA sequences. Nucleic Acids Res 20:1363-70
Burge, C; Campbell, A M; Karlin, S (1992) Over- and under-representation of short oligonucleotides in DNA sequences. Proc Natl Acad Sci U S A 89:1358-62
Campbell, A M (1992) Chromosomal insertion sites for phages and plasmids. J Bacteriol 174:7495-9
Pierson, D E; Campbell, A (1990) Cloning and nucleotide sequence of bisC, the structural gene for biotin sulfoxide reductase in Escherichia coli. J Bacteriol 172:2194-8
Limberger, R J; Campbell, A M (1987) Functional elements of DNA upstream from the integrase operon that are conserved in bacteriophages 434 and lambda. Gene 61:135-44
Redfield, R J; Campbell, A M (1987) Structure of cryptic lambda prophages. J Mol Biol 198:393-404

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