The long term goals are to understand how viruses interact with chromosomes and how genes for biosynthesis of proteins needed in small amounts are regulated. The health relatedness of these studies on prokaryotic model systems lies in the elucidation of basic mechanisms and in the perspective shed on natural biology of disease-causing viruses by the common features of bacterial and animal virology. The DNA of bacteriophage lambda upstream of the integrase operon will be examined for effects on integration and excision of two elements: a terminator-like sequence tI' that abuts the pI promoter and an open reading frame (ORF-55) upstream from it. Phage constructed to eliminate tI' will be examined for the effect of cII protein in the presence of transcription from pL, to test the hypothesis that tI' functions in the initial transition from the uncommitted to the lysogenic state. The ORF-55 gene expressed from a plasmid will be tested for complementation of the excisionase defect of the lambda crg phage, which has an IS2 insertion in ORF-55. Lambda crg prophages in inverted orientation will be tested for the ability to generate duplications by recombination with the defective gsr' prophage, and the defective prophages of E. coli strains of the ECOR collection will be compared. The properties of purified E. coli biotin sulfoxide reductase and the molecular basis for a newly evolved BDS reductase activity will be determined.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI008573-24
Application #
3124425
Study Section
Microbial Physiology and Genetics Subcommittee 2 (MBC)
Project Start
1978-06-01
Project End
1993-05-31
Budget Start
1991-06-01
Budget End
1992-05-31
Support Year
24
Fiscal Year
1991
Total Cost
Indirect Cost
Name
Stanford University
Department
Type
Schools of Arts and Sciences
DUNS #
800771545
City
Stanford
State
CA
Country
United States
Zip Code
94305
Campbell, A (1994) Comparative molecular biology of lambdoid phages. Annu Rev Microbiol 48:193-222
Campbell, A M (1993) Thirty years ago in Genetics: prophage insertion into bacterial chromosomes. Genetics 133:433-7
Campbell, A M (1993) Genome organization in prokaryotes. Curr Opin Genet Dev 3:837-44
Campbell, A M (1993) Co-chairman's remarks: genetic recombination in the molecular era. Gene 135:147-51
Campbell, A M (1992) Chromosomal insertion sites for phages and plasmids. J Bacteriol 174:7495-9
Karlin, S; Burge, C; Campbell, A M (1992) Statistical analyses of counts and distributions of restriction sites in DNA sequences. Nucleic Acids Res 20:1363-70
Burge, C; Campbell, A M; Karlin, S (1992) Over- and under-representation of short oligonucleotides in DNA sequences. Proc Natl Acad Sci U S A 89:1358-62
Pierson, D E; Campbell, A (1990) Cloning and nucleotide sequence of bisC, the structural gene for biotin sulfoxide reductase in Escherichia coli. J Bacteriol 172:2194-8
Limberger, R J; Campbell, A M (1987) Functional elements of DNA upstream from the integrase operon that are conserved in bacteriophages 434 and lambda. Gene 61:135-44
Redfield, R J; Campbell, A M (1987) Structure of cryptic lambda prophages. J Mol Biol 198:393-404

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