Abstract

The objective of the research is to characterize the eosinophil peroxidase system which consists of the granular enzyme eosinophil peroxidase, the cellular product hydrogen peroxide, and an oxidizable co-factor, supplied as iodide. An active peroxidase system has been identified in human and guinea pig peritoneal exudate eosinophils. Methods for measurement of hydrogen peroxide production by eosinophils have been developed. Anaerobic studies have shown that the peroxidase system does not participate in eosinophil - mediated damage of schistosomula. Further studies will explore hydrogen peroxide production, as well as functions of the peroxidase system.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI016432-07
Application #
3126652
Study Section
Immunological Sciences Study Section (IMS)
Project Start
1979-12-01
Project End
1987-05-31
Budget Start
1985-12-01
Budget End
1987-05-31
Support Year
7
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
Tufts University
Department
Type
Schools of Medicine
DUNS #
604483045
City
Boston
State
MA
Country
United States
Zip Code
02111

  Publications

Conway, B; Baskar, P; Bechtel, L J et al. (1992) Eosinophils as host cells for HIV-1. Arch Virol 127:373-7
Baskar, P; Pincus, S H (1992) Selective regulation of eosinophil degranulation by interleukin 1 beta. Proc Soc Exp Biol Med 199:249-54
Baskar, P; Silberstein, D S; Pincus, S H (1990) Inhibition of IgG-triggered human eosinophil function by IL-4. J Immunol 144:2321-6
Whitcomb, E A; Dinarello, C A; Pincus, S H (1989) Differential effects of interleukin-1 alpha and interleukin-1 beta on human peripheral blood eosinophils. Blood 73:1904-8
Whitcomb, E A; Wesolek, J H; Pincus, S H (1989) Modulation of human peripheral blood eosinophil function by tumor necrosis factor-alpha. Int Arch Allergy Appl Immunol 89:250-5
Baskar, P; Pincus, S H (1988) Oxidative requirement for degranulation of human peripheral blood eosinophils. Infect Immun 56:1907-11
Pincus, S H; Ramesh, K S; Wyler, D J (1987) Eosinophils stimulate fibroblast DNA synthesis. Blood 70:572-4
Pincus, S H (1986) Lectin binding by eosinophils. Proc Soc Exp Biol Med 182:344-9
Pincus, S H; Whitcomb, E A; Dinarello, C A (1986) Interaction of IL 1 and TPA in modulation of eosinophil function. J Immunol 137:3509-14
Ramesh, K S; Pincus, S H; Rocklin, R E (1985) Human lymphocyte-eosinophil interactions. I. Modulation of phytohemagglutinin-induced lymphocyte proliferation by eosinophils. Cell Immunol 92:366-75