Abstract

This project will attempt to elucidate the molecular basis for natural variation in resistance to phagocytosis by virulent group A Streptococci. Employing genetic and biochemical techniques the role of bacteriophage and chromosomal genes in the regulation of M protein synthesis will be investigated. Experimental data will further evaluate preliminary evidence that lysogenic conversion imparts resistance to phagocytosis. Comparison of restriction endonuclease maps of Bacteriophage DNA will extend our understanding of interactions between the virus and bacteria which result in resistance to phagocytosis.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI016722-06
Application #
3126787
Study Section
Bacteriology and Mycology Subcommittee 1 (BM)
Project Start
1981-09-01
Project End
1988-08-31
Budget Start
1986-09-01
Budget End
1987-08-31
Support Year
6
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
University of Minnesota Twin Cities
Department
Type
Schools of Medicine
DUNS #
168559177
City
Minneapolis
State
MN
Country
United States
Zip Code
55455

  Publications

Pritchard, K H; Cleary, P P (1996) Differential expression of genes in the vir regulon of Streptococcus pyogenes is controlled by transcription termination. Mol Gen Genet 250:207-13
McLandsborough, L A; Cleary, P P (1995) Insertional inactivation of virR in Streptococcus pyogenes M49 demonstrates that VirR functions as a positive regulator of ScpA, FcRA, OF, and M protein. FEMS Microbiol Lett 128:45-51
Cleary, P P; McLandsborough, L; Pritchard, K H (1995) Differential expression of genes in the vir regulon. Dev Biol Stand 85:145-8
Johansson, P J; Malone, C C; Williams Jr, R C et al. (1994) Streptococcus pyogenes type M12 protein shows selective binding to some human immunoglobulin G3 myeloma proteins. Infect Immun 62:3559-63
LaPenta, D; Rubens, C; Chi, E et al. (1994) Group A streptococci efficiently invade human respiratory epithelial cells. Proc Natl Acad Sci U S A 91:12115-9
La Penta, D; Zhang, X P; Cleary, P P (1994) Streptococcus pyogenes type IIa IgG Fc receptor expression is co-ordinately regulated with M protein and streptococcal C5a peptidase. Mol Microbiol 12:873-9
Cleary, P; Retnoningrum, D (1994) Group A streptococcal immunoglobulin-binding proteins: adhesins, molecular mimicry or sensory proteins? Trends Microbiol 2:131-6
Retnoningrum, D S; Cleary, P P (1994) M12 protein from Streptococcus pyogenes is a receptor for immunoglobulin G3 and human albumin. Infect Immun 62:2387-94
Retnoningrum, D S; Podbielski, A; Cleary, P P (1993) Type M12 protein from Streptococcus pyogenes is a receptor for IgG3. J Immunol 150:2332-40
Chen, C; Bormann, N; Cleary, P P (1993) VirR and Mry are homologous trans-acting regulators of M protein and C5a peptidase expression in group A streptococci. Mol Gen Genet 241:685-93

Showing the most recent 10 out of 27 publications