This project seeks to maintain, to develop and to expand a colony of mice which carry mutations of genes which affect skin graft transplantation. A substantial number of lines are already on hand, completed or under development. These include strains bearing H-2 mutations as well as non-H-2 gene mutations. New mutations will be sought by screening the progeny of ENU-treated or untreated male mice of strains C57BL/6Kh and BALB/cKh. In addition a number of other inbred, congenic and recombinant strains will be continually screened as part of a system to monitor their genetic integrity and mutants may be recovered from them as well. Mutants will be entered into immunogenetic analyses, both as part of formal collaborative efforts and by making them available to other investigators upon request. The formal collaborations include Dr. Stanley Nathenson (biochemical and structural analysis of mutant gene products at the protein and DNA levels), Dr. Ian McKenzie (serological analyses of mutant gene products), Dr. Kees Melief (functional consequences of the mutations for cell-mediated assays such as CML, MLR and MHC restriction), Dr. Peter Wettstein (looking for changes in integrated viral sequences as a result of mutations involving mutations of non-H-2 genes), and Dr. Byung Kim (assisting in electrophoretic techniques). We will be specifically examining the recently reovered H-2dm6 mutation (which has lost Dd, but retained Ld and apparently Rd as well) to see if D region products other than D,L and R can be identified which are missing in the mutant. We also propose attempting to raise alloantisera against some of the minor histocompatibility mutants as a step toward future analyses of these genes.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI016919-10
Application #
3126898
Study Section
Immunobiology Study Section (IMB)
Project Start
1979-09-01
Project End
1989-08-31
Budget Start
1988-09-01
Budget End
1989-08-31
Support Year
10
Fiscal Year
1988
Total Cost
Indirect Cost
Name
Northwestern University at Chicago
Department
Type
School of Medicine & Dentistry
DUNS #
005436803
City
Chicago
State
IL
Country
United States
Zip Code
60611
Wang, K; Busker-Mannie, A E; Hoeft, J et al. (2001) Prolonged Hya-disparate skin graft survival in ethanol-consuming mice: correlation with impaired delayed hypersensitivity. Alcohol Clin Exp Res 25:1542-8
Yun, T J; Melvold, R W; Pease, L R (1997) A complex major histocompatibility complex D locus variant generated by an unusual recombination mechanism in mice. Proc Natl Acad Sci U S A 94:1384-9
Makino, M; Murphy, D B; Melvold, R W et al. (1995) Impact of MHC class I gene on resistance to murine AIDS. Scand J Immunol 42:368-72
Rubocki, R J; Connolly, J M; Hansen, T H et al. (1991) Mutation at amino acid position 133 of H-2Dd prevents beta 2m association and immune recognition but not surface expression. J Immunol 146:2352-7
Busker, A E; Miller, S D; Melvold, R W (1990) Induction of allograft tolerance to the H-Y antigen in adult C57BL/6 mice: differential effects on delayed-type hypersensitivity and cytolytic T-lymphocyte activity. Cell Immunol 125:225-34
Melvold, R W; Jokinen, D M; Miller, S D et al. (1990) Identification of a locus on mouse chromosome 3 involved in differential susceptibility to Theiler's murine encephalomyelitis virus-induced demyelinating disease. J Virol 64:686-90
Melvold, R W; Jokinen, D M; Knobler, R L et al. (1987) Variations in genetic control of susceptibility to Theiler's murine encephalomyelitis virus (TMEV)-induced demyelinating disease. I. Differences between susceptible SJL/J and resistant BALB/c strains map near the T cell beta-chain constant gene on chromos J Immunol 138:1429-33
Colombo, M P; Melvold, R W; Wettstein, P J (1987) Inheritance of a mutant histocompatibility gene and a new mammary tumor virus genome in the B6.KH-84 mouse strain. Immunogenetics 26:99-104
Stuart, P M; Iannaccone, P M; Miller, S D et al. (1987) In vitro and in vivo correlates of hybrid tumor resistance. J Natl Cancer Inst 78:1159-68
Wettstein, P J; Melvold, R W (1986) Xenotropic virus-related restriction patterns of non-H-2 histocompatibility mutant mice strains. Immunogenetics 23:156-63

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