We plan to isolate the genes which encode the murine T-cell receptor for antigen. We will employ three independent approaches. First, we will screen libraries containing T-cell DNA cloned into an expression vector with serological reagents to detect clones expressing the cell-surface markers I-J, Tsu, and CGAT(an idiotypic determinant). Second, we will determine the amino acid sequence of polypeptides from factors secreted by T-suppressor hybridomas. These protein sequences will be reverse translated into DNA sequence and synthetic DNA probes will be synthesized and used as primers or as DNA probes to screen appropriate cDNA libraries. Finally, we intend to identify gene sequences in cloned T cells which undergo DNA rearrangements. Some of these rearranged sequences may be T-cell receptor genes. Once DNA probes for the T-cell receptor have been obtained, we will characterize the corresponding genomic clones with regard to structure, organization, rearrangements, and diversification.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI017565-05
Application #
3127275
Study Section
Immunobiology Study Section (IMB)
Project Start
1981-01-01
Project End
1986-12-31
Budget Start
1985-01-01
Budget End
1985-12-31
Support Year
5
Fiscal Year
1985
Total Cost
Indirect Cost
Name
California Institute of Technology
Department
Type
Schools of Arts and Sciences
DUNS #
078731668
City
Pasadena
State
CA
Country
United States
Zip Code
91125
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