Abstract

We plan to isolate the genes which encode the murine T-cell receptor for antigen. We will employ three independent approaches. First, we will screen libraries containing T-cell DNA cloned into an expression vector with serological reagents to detect clones expressing the cell-surface markers I-J, Tsu, and CGAT(an idiotypic determinant). Second, we will determine the amino acid sequence of polypeptides from factors secreted by T-suppressor hybridomas. These protein sequences will be reverse translated into DNA sequence and synthetic DNA probes will be synthesized and used as primers or as DNA probes to screen appropriate cDNA libraries. Finally, we intend to identify gene sequences in cloned T cells which undergo DNA rearrangements. Some of these rearranged sequences may be T-cell receptor genes. Once DNA probes for the T-cell receptor have been obtained, we will characterize the corresponding genomic clones with regard to structure, organization, rearrangements, and diversification.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI017565-06
Application #
3127276
Study Section
Immunobiology Study Section (IMB)
Project Start
1981-01-01
Project End
1986-12-31
Budget Start
1986-01-01
Budget End
1986-12-31
Support Year
6
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
California Institute of Technology
Department
Type
Schools of Arts and Sciences
DUNS #
078731668
City
Pasadena
State
CA
Country
United States
Zip Code
91125

  Publications

Edidin, M; Stroynowski, I (1991) Differences between the lateral organization of conventional and inositol phospholipid-anchored membrane proteins. A further definition of micrometer scale membrane domains. J Cell Biol 112:1143-50
Ulker, N; Lewis, K D; Hood, L E et al. (1990) Activated T cells transcribe an alternatively spliced mRNA encoding a soluble form of Qa-2 antigen. EMBO J 9:3839-47
Stroynowski, I (1990) Molecules related to class-I major histocompatibility complex antigens. Annu Rev Immunol 8:501-30
Brorson, K A; Hunt 3rd, S W; Hunkapiller, T et al. (1989) Comparison of exon 5 sequences from 35 class I genes of the BALB/c mouse. J Exp Med 170:1837-58
Brorson, K A; Richards, S; Hunt 3rd, S W et al. (1989) Analysis of a new class I gene mapping to the Hmt region of the mouse. Immunogenetics 30:273-83
Klotz, J L; Barth, R K; Kiser, G L et al. (1989) Restriction fragment length polymorphisms of the mouse T-cell receptor gene families. Immunogenetics 29:191-201
Woolf, T; Lai, E; Kronenberg, M et al. (1988) Mapping genomic organization by field inversion and two-dimensional gel electrophoresis: application to the murine T-cell receptor gamma gene family. Nucleic Acids Res 16:3863-75
Lai, E; Barth, R K; Hood, L (1987) Genomic organization of the mouse T-cell receptor beta-chain gene family. Proc Natl Acad Sci U S A 84:3846-50
Haars, R; Kronenberg, M; Gallatin, W M et al. (1986) Rearrangement and expression of T cell antigen receptor and gamma genes during thymic development. J Exp Med 164:1-24
Yancopoulos, G D; Blackwell, T K; Suh, H et al. (1986) Introduced T cell receptor variable region gene segments recombine in pre-B cells: evidence that B and T cells use a common recombinase. Cell 44:251-9

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