Attempts will be made to identify the staphylocidal fatty acid (SFA) produced in staphylococcal abscesses. A newly detected fatty acid-modifying enzyme (FAME) will be purified and characterized. Antibodies to FAME will be examined to determine whether they are protective in the host. S. aureus strains will be examined to establish the incidence of FAME producing strains, and the amount of FAME produced in abscesses will be measured. Antibodies to staphylococcal lipase will be prepared and evaluated for their protective ability. Lipase-negative mutants of S. aureus will be compared with their parent strains for their ability to survive in abscesses. The production of lipase within abscesses will also be measured. The staphylococcal factor which can stimulate lipid accumulation in macrophages will be purified and characterized. We also intend to see whether macrophages are the source of SEA and triglyerides. Studies will be conducted to determine whether diabetes, steroid administration, ether inhalation, or delayed hypersenitivity can alter SFA or glceride production in abscesses. A competitive radioimmunoassay will be developed to measure the amount of S. aureus delta toxin produced in abscesses.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI019879-03
Application #
3129318
Study Section
Bacteriology and Mycology Subcommittee 1 (BM)
Project Start
1983-12-01
Project End
1987-05-31
Budget Start
1985-12-01
Budget End
1987-05-31
Support Year
3
Fiscal Year
1986
Total Cost
Indirect Cost
Name
Ohio State University
Department
Type
Schools of Medicine
DUNS #
098987217
City
Columbus
State
OH
Country
United States
Zip Code
43210
Xiong, Z; Ge, S; Chamberlain, N R et al. (1993) Growth cycle-induced changes in sensitivity of Staphylococcus aureus to bactericidal lipids from abscesses. J Med Microbiol 39:58-63
Shryock, T R; Kapral, F A (1992) The production of bactericidal fatty acids from glycerides in staphylococcal abscesses. J Med Microbiol 36:288-92
Engler, H D; Kapral, F A (1992) The production of a bactericidal monoglyceride in staphylococcal abscesses. J Med Microbiol 37:238-44
Xiong, Z; Kapral, F A (1992) Carotenoid pigment levels in Staphylococcus aureus and sensitivity to oleic acid. J Med Microbiol 37:192-4
Long, J P; Hart, J; Albers, W et al. (1992) The production of fatty acid modifying enzyme (FAME) and lipase by various staphylococcal species. J Med Microbiol 37:232-4
Mortensen, J E; Shryock, T R; Kapral, F A (1992) Modification of bactericidal fatty acids by an enzyme of Staphylococcus aureus. J Med Microbiol 36:293-8
Kapral, F A; Smith, S; Lal, D (1992) The esterification of fatty acids by Staphylococcus aureus fatty acid modifying enzyme (FAME) and its inhibition by glycerides. J Med Microbiol 37:235-7
Shryock, T R; Dye, E S; Kapral, F A (1992) The accumulation of bactericidal lipids in staphylococcal abscesses. J Med Microbiol 36:332-6
Mortensen, J E; Kapral, F A (1992) Effect of capsulation on the resistance of Staphylococcus aureus to the bactericidal lipids produced in abscesses. J Med Microbiol 36:337-40
Chamberlain, N R; Mehrtens, B G; Xiong, Z et al. (1991) Correlation of carotenoid production, decreased membrane fluidity, and resistance to oleic acid killing in Staphylococcus aureus 18Z. Infect Immun 59:4332-7