This project will study the production of the fatty acid modifying enzyme (FAME) by Staphylococcus aureus strains within abscesses. It will determine whether antibodies to FAME or lipase are protective for the host. The project will also determine whether lipase-negative mutants of S. aureus are less able to survive in abscesses. Studies will be done to determine whether diabetes, steroid administration, ether inhalation, or delayed hypersensitivity will alter the host's ability to synthesize the bactericidal lipids in abscesses. Attempts will be made to isolate the substance from Candida albicans that can increase the host's susceptibility to S. aureus. Efforts will be made to determine whether granulocytes or macrophages are involved in the synthesis of the bactericidal lipids and whether the conditions existing in inflammatory sites are required for such synthesis. Studies will also be conducted to see whether the resistance to reinfection with S. aureus can be transfered by lymphoid cells.

National Institute of Health (NIH)
National Institute of Allergy and Infectious Diseases (NIAID)
Research Project (R01)
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Bacteriology and Mycology Subcommittee 1 (BM)
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Ohio State University
Schools of Medicine
United States
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Xiong, Z; Ge, S; Chamberlain, N R et al. (1993) Growth cycle-induced changes in sensitivity of Staphylococcus aureus to bactericidal lipids from abscesses. J Med Microbiol 39:58-63
Shryock, T R; Kapral, F A (1992) The production of bactericidal fatty acids from glycerides in staphylococcal abscesses. J Med Microbiol 36:288-92
Engler, H D; Kapral, F A (1992) The production of a bactericidal monoglyceride in staphylococcal abscesses. J Med Microbiol 37:238-44
Xiong, Z; Kapral, F A (1992) Carotenoid pigment levels in Staphylococcus aureus and sensitivity to oleic acid. J Med Microbiol 37:192-4
Long, J P; Hart, J; Albers, W et al. (1992) The production of fatty acid modifying enzyme (FAME) and lipase by various staphylococcal species. J Med Microbiol 37:232-4
Mortensen, J E; Shryock, T R; Kapral, F A (1992) Modification of bactericidal fatty acids by an enzyme of Staphylococcus aureus. J Med Microbiol 36:293-8
Kapral, F A; Smith, S; Lal, D (1992) The esterification of fatty acids by Staphylococcus aureus fatty acid modifying enzyme (FAME) and its inhibition by glycerides. J Med Microbiol 37:235-7
Shryock, T R; Dye, E S; Kapral, F A (1992) The accumulation of bactericidal lipids in staphylococcal abscesses. J Med Microbiol 36:332-6
Mortensen, J E; Kapral, F A (1992) Effect of capsulation on the resistance of Staphylococcus aureus to the bactericidal lipids produced in abscesses. J Med Microbiol 36:337-40
Chamberlain, N R; Mehrtens, B G; Xiong, Z et al. (1991) Correlation of carotenoid production, decreased membrane fluidity, and resistance to oleic acid killing in Staphylococcus aureus 18Z. Infect Immun 59:4332-7