A detailed analysis of the structue and organization of the important family of genes that encode anti-DNA autoantibodies will enhance our understanding of the role of the immunoglobulin V (variable) gene repertoire in the pathogenesis of autoimmune disease. Using a recently developed panel of anti-DNA autoantibody producing hybridomas derived from NZB/NZW F1 mice, and applying recent advances in recombinant DNA technology, I propose to: a) Clone and sequence several anti-DNA autoantibody VH and VL genes. b) Determine the number of germline VH and VL gene segments contributing to the autoimmune response. c) Determine whether polymorphisms in the germline repertoire of these genes are at all associated with the development of autoimmune disease. d) Determine the linkage relationships among the V gene segments encoding the anti-DNA autoantibodies and their location relative to other V gene loci.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI024329-03
Application #
3137296
Study Section
Mammalian Genetics Study Section (MGN)
Project Start
1986-04-01
Project End
1989-03-31
Budget Start
1988-04-01
Budget End
1989-03-31
Support Year
3
Fiscal Year
1988
Total Cost
Indirect Cost
Name
University of California Los Angeles
Department
Type
Schools of Medicine
DUNS #
119132785
City
Los Angeles
State
CA
Country
United States
Zip Code
90095