The investigators propose to develop retroviral derivatives capable of integrating into preselected DNA sites. They have previously reported the construction and characterization of a fusion protein composed of HIV integrase linked to the site specific DNA-binding domain of l repressor. This hybrid protein directed integration selectively to target DNAs containing sites recognized by the repressor domain. More recently they have found that i) they can create biologically active HIV proviruses encoding integrases fused to DNA-binding domains, and ii) preintegration complexes containing such fusions direct integration preferentially into target DNA containing appropriate sites.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
2R01AI037489-03
Application #
2004219
Study Section
AIDS and Related Research Study Section 3 (ARRC)
Project Start
1996-12-01
Project End
2000-11-30
Budget Start
1996-12-01
Budget End
1997-11-30
Support Year
3
Fiscal Year
1997
Total Cost
Indirect Cost
Name
Salk Institute for Biological Studies
Department
Type
DUNS #
005436803
City
La Jolla
State
CA
Country
United States
Zip Code
92037