Abstract

The overall goal of this application is targeted methylation of the HIV LTR promoter in a manner such that HIV transcription is silenced without adverse effects on the host. This will be achieved not by influencing the affinity of transcriptional factors for the promoter, but rather the transcribing enzyme, i.e., RNA polymerase, fails to productively use methylated DNA.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI040021-03
Application #
2672793
Study Section
AIDS and Related Research Study Section 3 (ARRC)
Project Start
1996-07-15
Project End
1999-06-30
Budget Start
1998-07-01
Budget End
1999-06-30
Support Year
3
Fiscal Year
1998
Total Cost
Indirect Cost

  Institution

Name
Columbia University (N.Y.)
Department
Genetics
Type
Schools of Medicine
DUNS #
167204994
City
New York
State
NY
Country
United States
Zip Code
10032

  Publications

Xu, G L; Bestor, T H (1997) Cytosine methylation targetted to pre-determined sequences. Nat Genet 17:376-8
Yoder, J A; Bestor, T H (1996) Genetic analysis of genomic methylation patterns in plants and mammals. Biol Chem 377:605-10