Abstract

The decay of HIV and turnover of CD4+ lymphocytes have been described recently as being very rapid suggesting that HIV replication is highly productive and that the turnover of CD4+ cells is high. This application is to extend these observations.
Specific Aim 1 is to define the kinetics of decay of HIV or SIV in the compartments containing HIV by using antiviral agents.
The Second Aim i s to define where SIV is cleared and produced in macaques.
The Third Aim i s to characterize the types of lymphocytes before and after antivirals and to determine the mechanism of their regeneration.
The Fourth Aim i s to compare populations of HIV+ people for lymphocyte subpopulations by examining the telomeric length in sequential samples of CD4+ and CD8+ lymphocytes. The Fifth Aim is to examine CD4 and CD8 lymphocytic tissue/turnover using BrdU studies in monkeys with or without SIV infection.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI040387-04
Application #
2887314
Study Section
AIDS and Related Research Study Section 3 (ARRC)
Program Officer
Kagan, Jonathan M
Project Start
1996-08-15
Project End
2001-08-31
Budget Start
1999-09-01
Budget End
2000-08-31
Support Year
4
Fiscal Year
1999
Total Cost
Indirect Cost

  Institution

Name
Aaron Diamond AIDS Research Center
Department
Type
DUNS #
786658872
City
New York
State
NY
Country
United States
Zip Code
10016

  Publications

Di Mascio, Michele; Percus, Jerome K; Percus, Ora E et al. (2005) Duration of an intermittent episode of viremia. Bull Math Biol 67:885-900
Ramratnam, Bharat; Ribeiro, Ruy; He, Tian et al. (2004) Intensification of antiretroviral therapy accelerates the decay of the HIV-1 latent reservoir and decreases, but does not eliminate, ongoing virus replication. J Acquir Immune Defic Syndr 35:33-7
De Boer, Rob J; Mohri, Hiroshi; Ho, David D et al. (2003) Estimating average cellular turnover from 5-bromo-2'-deoxyuridine (BrdU) measurements. Proc Biol Sci 270:849-58
Percus, Jerome K; Percus, Ora E; Markowitz, Martin et al. (2003) The distribution of viral blips observed in HIV-1 infected patients treated with combination antiretroviral therapy. Bull Math Biol 65:263-77
De Boer, Rob J; Mohri, Hiroshi; Ho, David D et al. (2003) Turnover rates of B cells, T cells, and NK cells in simian immunodeficiency virus-infected and uninfected rhesus macaques. J Immunol 170:2479-87
Zhang, Linqi; Rowe, Leslie; He, Tian et al. (2002) Compartmentalization of surface envelope glycoprotein of human immunodeficiency virus type 1 during acute and chronic infection. J Virol 76:9465-73
Ribeiro, Ruy M; Mohri, Hiroshi; Ho, David D et al. (2002) In vivo dynamics of T cell activation, proliferation, and death in HIV-1 infection: why are CD4+ but not CD8+ T cells depleted? Proc Natl Acad Sci U S A 99:15572-7
Zhang, Linqi; Dailey, Peter J; Gettie, Agegnehu et al. (2002) The liver is a major organ for clearing simian immunodeficiency virus in rhesus monkeys. J Virol 76:5271-3
Foster, J L; Molina, R P; Luo, T et al. (2001) Genetic and functional diversity of human immunodeficiency virus type 1 subtype B Nef primary isolates. J Virol 75:1672-80
Mohri, H; Perelson, A S; Tung, K et al. (2001) Increased turnover of T lymphocytes in HIV-1 infection and its reduction by antiretroviral therapy. J Exp Med 194:1277-87

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