Abstract

The goal of this application is to develop a transmission blocking vaccine to Plasmodium falciparum. This vaccine is meant to induce antibodies which bind to antigens present on the surface of male and female gameites and parasites developing in the mosquito midgut. The main candidate antigen is Pfs25, a surface protein that is expressed at the onset of gametogenesis. The vaccine will be based on DNA vaccines given either alone or in a prime-boast regimen with protein vaccines.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI047089-04
Application #
6632235
Study Section
Special Emphasis Panel (ZRG1-TMP (03))
Program Officer
Wali, Tonu M
Project Start
2000-03-01
Project End
2006-02-28
Budget Start
2003-03-01
Budget End
2006-02-28
Support Year
4
Fiscal Year
2003
Total Cost
$301,600
Indirect Cost

  Institution

Name
Johns Hopkins University
Department
Microbiology/Immun/Virology
Type
Schools of Public Health
DUNS #
001910777
City
Baltimore
State
MD
Country
United States
Zip Code
21218

  Publications

Datta, Dibyadyuti; Bansal, Geetha P; Grasperge, Brooke et al. (2017) Comparative functional potency of DNA vaccines encoding Plasmodium falciparum transmission blocking target antigens Pfs48/45 and Pfs25 administered alone or in combination by in vivo electroporation in rhesus macaques. Vaccine 35:7049-7056
Heidari, Zahra; Arora, Jaspreet S; Datta, Dibyadyuti et al. (2017) Impact of the Charge Ratio on the In Vivo Immunogenicity of Lipoplexes. Pharm Res 34:1796-1804
Datta, Dibyadyuti; Bansal, Geetha P; Gerloff, Dietlind L et al. (2017) Immunogenicity and malaria transmission reducing potency of Pfs48/45 and Pfs25 encoded by DNA vaccines administered by intramuscular electroporation. Vaccine 35:264-272
Kumar, Nirbhay (2017) Modulation of transmission success of Plasmodium falciparum gametocytes (sexual stages) in various species of Anopheles by erythrocytic asexual stage parasites. Acta Trop 176:263-269
Bansal, Geetha P; Weinstein, Corey S; Kumar, Nirbhay (2016) Insight into phagocytosis of mature sexual (gametocyte) stages of Plasmodium falciparum using a human monocyte cell line. Acta Trop 157:96-101
Kumar, Rajesh; Ray, Paresh C; Datta, Dibyadyuti et al. (2015) Nanovaccines for malaria using Plasmodium falciparum antigen Pfs25 attached gold nanoparticles. Vaccine 33:5064-71
Kumar, Rajesh; Ledet, Grace; Graves, Richard et al. (2015) Potent Functional Immunogenicity of Plasmodium falciparum Transmission-Blocking Antigen (Pfs25) Delivered with Nanoemulsion and Porous Polymeric Nanoparticles. Pharm Res 32:3827-36
Datta, Dibyadyuti; Bansal, Geetha P; Kumar, Rajesh et al. (2015) Evaluation of the Impact of Codon Optimization and N-Linked Glycosylation on Functional Immunogenicity of Pfs25 DNA Vaccines Delivered by In Vivo Electroporation in Preclinical Studies in Mice. Clin Vaccine Immunol 22:1013-9
Lukianova-Hleb, Ekaterina; Bezek, Sarah; Szigeti, Reka et al. (2015) Transdermal Diagnosis of Malaria Using Vapor Nanobubbles. Emerg Infect Dis 21:1122-7
Kumar, Rajesh; Angov, Evelina; Kumar, Nirbhay (2014) Potent malaria transmission-blocking antibody responses elicited by Plasmodium falciparum Pfs25 expressed in Escherichia coli after successful protein refolding. Infect Immun 82:1453-9

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