Evidence of simian immunodeficiency virus (SIV) infection has been reported for 27 different species of African non-human primates. Two of these viruses, SIVcpz from chimpanzees and SIVsm from sooty mangabeys, are the cause of AIDS in humans. Together, they have been transmitted to humans on at least eight occasions. An important scientific and public health concern thus is whether and to what extent humans are exposed to additional SIV strains (besides SIVsm and SIVcpz), and whether such exposure has led to zoonotic transmission. An additional, more fundamental question pertains to the spectrum of SIV diversity that exists in wild-living African primates and the ecological niche occupied by this broad family of primate lentiviruses. In this grant application, we propose to address these questions in Cameroon, a country known to lie within the center of HIV-1 groups M, N, and O endemicity and to harbor a diverse set of SIV- infected non-human primates. We have assembled a research team of co-investigators, with each member of the team bringing essential expertise, unique capability, and work scope to the project. On the basis of published work and research in progress, we demonstrate that our goals are scientifically and technically feasible and that our team is uniquely positioned to accomplish them.
Specific aims of the project are: 1. To determine the full spectrum of SIV infected non-human primates in Cameroon. We will use serologic and PCR-based approaches to test primate bushmeat confiscated by the Ministry of Forest and Environment as well as fecal and urine samples collected from wild apes for SIV specific antibody and nucleic acids. 2. To determine the genetic and phylogenetic identity of all SIV lineages infecting primate species in Cameroon. We will PCR amplify, clone and sequence full-length genomes for all major SIV lineages, and construct replication competent proviral clones for basic biological and immunological studies. 3. To determine the prevalence of SIV infection in wild-living primates in Cameroon. We will develop sensitive, lineage specific serological and PCR based assays and apply them to determine the prevalence of SIV in each naturally infected primate species. 4. To assess the prevalence of SIV infection in human populations from Cameroon and surrounding areas of west central Africa. We will use novel screening and confirmatory assays to analyze existing serum banks from west equatorial human populations for evidence of SIV infection. We expect these studies to yield a complete record of all naturally infected primate species in Cameroon, an accurate assessment of their levels of infection, and a first approximation of human zoonotic risk.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI050529-02
Application #
6511656
Study Section
AIDS and Related Research 8 (AARR)
Program Officer
Young, Janet M
Project Start
2001-08-01
Project End
2006-05-31
Budget Start
2002-06-01
Budget End
2003-05-31
Support Year
2
Fiscal Year
2002
Total Cost
$578,250
Indirect Cost
Name
University of Alabama Birmingham
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
004514360
City
Birmingham
State
AL
Country
United States
Zip Code
35294
Barbian, Hannah J; Li, Yingying; Ramirez, Miguel et al. (2018) Destabilization of the gut microbiome marks the end-stage of simian immunodeficiency virus infection in wild chimpanzees. Am J Primatol 80:
D'arc, Mirela; Furtado, Carolina; Siqueira, Juliana D et al. (2018) Assessment of the gorilla gut virome in association with natural simian immunodeficiency virus infection. Retrovirology 15:19
Ahuka-Mundeke, Steve; Mbala-Kingebeni, Placide; Ndimbo-Kumogo, Simon-Pierre et al. (2017) Full Genome Characterization of a New Simian Immune Deficiency Virus Lineage in a Naturally Infected Cercopithecus ascanius whitesidei in the Democratic Republic of Congo Reveals High Genetic Diversity Among Red-Tailed Monkeys in Central and Eastern Afric AIDS Res Hum Retroviruses 33:735-739
Steve, Ahuka-Mundeke; Ahidjo, Ayouba; Placide, Mbala-Kingebeni et al. (2017) High Prevalences and a Wide Genetic Diversity of Simian Retroviruses in Non-human Primate Bushmeat in Rural Areas of the Democratic Republic of Congo. Ecohealth 14:100-114
Clarke, Erik L; Sundararaman, Sesh A; Seifert, Stephanie N et al. (2017) swga: a primer design toolkit for selective whole genome amplification. Bioinformatics 33:2071-2077
Ayouba, Ahidjo; Michem, Annelies; Peeters, Martine et al. (2017) Full-Genome Characterization of Simian T-Cell Leukemia Virus Type 1 Subtype b from a Wild-Born Captive Gorilla gorilla gorilla with T-Cell Lymphoma. Genome Announc 5:
Moeller, Andrew H; Caro-Quintero, Alejandro; Mjungu, Deus et al. (2016) Cospeciation of gut microbiota with hominids. Science 353:380-2
Guichet, Emilande; Aghokeng, Avelin; Eymard-Duvernay, Sabrina et al. (2016) Field evaluation of an open and polyvalent universal HIV-1/SIVcpz/SIVgor quantitative RT-PCR assay for HIV-1 viral load monitoring in comparison to Abbott RealTime HIV-1 in Cameroon. J Virol Methods 237:121-126
Heigele, Anke; Kmiec, Dorota; Regensburger, Kerstin et al. (2016) The Potency of Nef-Mediated SERINC5 Antagonism Correlates with the Prevalence of Primate Lentiviruses in the Wild. Cell Host Microbe 20:381-391
Fünfstück, Tillmann; Arandjelovic, Mimi; Morgan, David B et al. (2015) The sampling scheme matters: Pan troglodytes troglodytes and P. t. schweinfurthii are characterized by clinal genetic variation rather than a strong subspecies break. Am J Phys Anthropol 156:181-91

Showing the most recent 10 out of 97 publications