The object of the proposed reseach is to determine the parameters of a capacitively coupled electric field required to produce long term growth stimulation in the lamb proximal radial growth plate as measured roentgenograhically with the aid of tantalum metaphyseal markers. A second objective is to determine the effect of a capacitively coupled electrical field on intracellular ionized calcium homeostasis, prostaglandin synthesis, and collagen synthesis and type in bovine growth plate chondrocytes grown in pellet form and in monolayer, as well as cytoplasmic components as determined by point-counting analysis of electron micrographs of rat costochondral junctions grown in organ culture. The long term objective is to develop a non-invasive method of stimulating the epiphyseal growth plate to accelerated growth in those children with limb length inequality.
| Conzemius, M G; Smith, G K; Brighton, C T et al. (1994) Analysis of physeal growth in dogs, using biplanar radiography. Am J Vet Res 55:22-7 |
| Clark, C C; Tolin, B S; Brighton, C T (1991) The effect of oxygen tension on proteoglycan synthesis and aggregation in mammalian growth plate chondrocytes. J Orthop Res 9:477-84 |
| Iannotti, J P; Brighton, C T; Stambough, J E (1989) Subcellular regulation of the ionized calcium pool in isolated growth-plate chondrocytes. Clin Orthop Relat Res :285-93 |
| Iannotti, J P; Brighton, C T (1989) Cytosolic ionized calcium concentration in isolated chondrocytes from each zone of the growth plate. J Orthop Res 7:511-8 |
