During the development of muscle, proliferating myogenic cells differentiate and fuse to form multinucleate cells that synthesize proteins necessary for the assembly of striated myofibrils. One event previously shown by the applicant to be critical to this multistep process is the specific, Ca++-dependent adhesion of myoblasts prior to their fusion. The work proposed here aims to isolate and identify the cell surface molecules mediating myoblast recognition using a combined biochemical and immunological approach. Recent work by the applicant indicates that the cell surface adhesion molecules are glycoproteins. Adhesion glycoproteins are currently being purified from pectoral muscle explants and myoblast cultures using anion exchange and lectin affinity chromomatrophy. To date our active fraction contains eight glycoproteins. We are raising monoclonal and polyclonal, monospecific antibodies against these glycoproteins in an effort to isolate antibodies that inhibit Ca++-dependent myoblast adhesion. Monospecific, adhesion-perturbing antibodies will be used to identify and localize the adhesion glycoproteins and to study their expression during myogenesis.

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Research Project (R01)
Project #
1R01AR037945-01
Application #
3158338
Study Section
Cellular Biology and Physiology Subcommittee 1 (CBY)
Project Start
1987-03-01
Project End
1992-02-29
Budget Start
1987-03-01
Budget End
1988-02-29
Support Year
1
Fiscal Year
1987
Total Cost
Indirect Cost
Name
Lankenau Hospital
Department
Type
DUNS #
City
Wynnewood
State
PA
Country
United States
Zip Code
19096
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