Abstract

During the development of muscle, proliferating myogenic cells differentiate and fuse to form multinucleate cells that synthesize proteins necessary for the assembly of striated myofibrils. One event previously shown by the applicant to be critical to this multistep process is the specific, Ca++-dependent adhesion of myoblasts prior to their fusion. The work proposed here aims to isolate and identify the cell surface molecules mediating myoblast recognition using a combined biochemical and immunological approach. Recent work by the applicant indicates that the cell surface adhesion molecules are glycoproteins. Adhesion glycoproteins are currently being purified from pectoral muscle explants and myoblast cultures using anion exchange and lectin affinity chromomatrophy. To date our active fraction contains eight glycoproteins. We are raising monoclonal and polyclonal, monospecific antibodies against these glycoproteins in an effort to isolate antibodies that inhibit Ca++-dependent myoblast adhesion. Monospecific, adhesion-perturbing antibodies will be used to identify and localize the adhesion glycoproteins and to study their expression during myogenesis.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Research Project (R01)
Project #
5R01AR037945-04
Application #
3158345
Study Section
Cellular Biology and Physiology Subcommittee 1 (CBY)
Project Start
1987-03-01
Project End
1992-02-29
Budget Start
1990-03-01
Budget End
1991-02-28
Support Year
4
Fiscal Year
1990
Total Cost
Indirect Cost

  Institution

Name
Lankenau Hospital
Department
Type
DUNS #
City
Wynnewood
State
PA
Country
United States
Zip Code
19096

  Publications

Soler, A P; Knudsen, K A (1994) N-cadherin involvement in cardiac myocyte interaction and myofibrillogenesis. Dev Biol 162:9-17
Johnson, K R; Lewis, J E; Li, D et al. (1993) P- and E-cadherin are in separate complexes in cells expressing both cadherins. Exp Cell Res 207:252-60
Soler, A P; Johnson, K R; Wheelock, M J et al. (1993) Rhabdomyosarcoma-derived cell lines exhibit aberrant expression of the cell-cell adhesion molecules N-CAM, N-cadherin, and cadherin-associated proteins. Exp Cell Res 208:84-93
Knudsen, K A; Wheelock, M J (1992) Plakoglobin, or an 83-kD homologue distinct from beta-catenin, interacts with E-cadherin and N-cadherin. J Cell Biol 118:671-9
Soler, A P; Knudsen, K A (1991) Colocalization of N-CAM and N-cadherin in avian skeletal myoblasts. Dev Biol 148:389-92
Wheelock, M J; Knudsen, K A (1991) N-cadherin-associated proteins in chicken muscle. Differentiation 46:35-42
Wheelock, M J; Knudsen, K A (1991) Cadherins and associated proteins. In Vivo 5:505-13
Soszka, T; Knudsen, K A; Beviglia, L et al. (1991) Inhibition of murine melanoma cell-matrix adhesion and experimental metastasis by albolabrin, an RGD-containing peptide isolated from the venom of Trimeresurus albolabris. Exp Cell Res 196:6-12
Rucinski, B; Niewiarowski, S; Holt, J C et al. (1990) Batroxostatin, an Arg-Gly-Asp-containing peptide from Bothrops atrox, is a potent inhibitor of platelet aggregation and cell interaction with fibronectin. Biochim Biophys Acta 1054:257-62
Knudsen, K A (1990) Cell adhesion molecules in myogenesis. Curr Opin Cell Biol 2:902-6

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