Abstract

This proposal is aimed at isolating and purifying a molecule obtained from human heterotopic bone. This molecule stimulates many of the cell parameters associated with bone formation. In the Specific Aims of this grant we propose to examine the phenotypic changes which occur in bone and cartilage cells after exposure to the molecule and to characterize the interactions of other one target hormones with the factor. We will also determine if the stimulatory molecule obtained from a pathological tissue if the same as the molecule obtained from normal tissue.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Research Project (R01)
Project #
5R01AR038618-02
Application #
3158662
Study Section
Orthopedics and Musculoskeletal Study Section (ORTH)
Project Start
1988-02-01
Project End
1991-01-31
Budget Start
1989-02-01
Budget End
1990-01-31
Support Year
2
Fiscal Year
1989
Total Cost
Indirect Cost

  Institution

Name
University of Rochester
Department
Type
School of Medicine & Dentistry
DUNS #
208469486
City
Rochester
State
NY
Country
United States
Zip Code
14627

  Publications

Rosier, R N; O'Keefe, R J; Teot, L A et al. (1997) P-glycoprotein expression in cartilaginous tumors. J Surg Oncol 65:95-105
Hicks, D G; Judkins, A R; Sickel, J Z et al. (1996) Granular histiocytosis of pelvic lymph nodes following total hip arthroplasty. The presence of wear debris, cytokine production, and immunologically activated macrophages. J Bone Joint Surg Am 78:482-96
Martinez, D A; Zuscik, M J; Ishibe, M et al. (1995) Identification of functional insulin-like growth factor-II/mannose-6-phosphate receptors in isolated bone cells. J Cell Biochem 59:246-57
O'Keefe, R J; Crabb, I D; Puzas, J E et al. (1994) Effects of transforming growth factor-beta 1 and fibroblast growth factor on DNA synthesis in growth plate chondrocytes are enhanced by insulin-like growth factor-I. J Orthop Res 12:299-310
Loveys, L S; Gelb, D; Hurwitz, S R et al. (1993) Effects of parathyroid hormone-related peptide on chick growth plate chondrocytes. J Orthop Res 11:884-91
Crabb, I D; Hughes, S S; Hicks, D G et al. (1992) Nonradioactive in situ hybridization using digoxigenin-labeled oligonucleotides. Applications to musculoskeletal tissues. Am J Pathol 141:579-89
O'Keefe, R J; Crabb, I D; Puzas, J E et al. (1992) Influence of prostaglandins on DNA and matrix synthesis in growth plate chondrocytes. J Bone Miner Res 7:397-404
Ishibe, M; Rosier, R N; Puzas, J E (1991) Activation of osteoblast insulin-like growth factor-II/cation-independent mannose-6-phosphate receptors by specific phosphorylated sugars and antibodies induce insulin-like growth factor-II effects. Endocr Res 17:357-66
Ishibe, M; Rosier, R N; Puzas, J E (1991) Human prostatic acid phosphatase directly stimulates collagen synthesis and alkaline phosphatase content of isolated bone cells. J Clin Endocrinol Metab 73:785-92
Sawyer, J R; Myers, M A; Rosier, R N et al. (1991) Heterotopic ossification: clinical and cellular aspects. Calcif Tissue Int 49:208-15

Showing the most recent 10 out of 15 publications