B cells in germinal centers will undergo apoptosis unless they interact with a cognate T cell. This interaction is critical in rescuing specific B cells and shaping the serologic response to antigen. In systemic lupus, B cells secreting anti-DNA antibodies appear to proliferate in germinal centers and escape deletion by interaction with cognate T cells that recognized a Class II histocompatibility molecule with its associated peptide on the autoreactive B cell. We producing B cells to identify we propose to isolate peptides of immunoglobulin variable regions or the DNA binding proteins that may be recognized by T cells in germinal centers. Concurrently, as an integral part of this study we propose to isolate T cells that are activated by the Class II-peptide complex on anti-DNA producing B cells. These studies should help dissect at a molecular level the B cell processing and B and T cell interactions that permit the production of pathogenic anti-DNA antibodies in systemic lupus.

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Research Project (R01)
Project #
5R01AR042533-04
Application #
2006350
Study Section
Arthritis and Musculoskeletal and Skin Diseases Special Grants Review Committee (AMS)
Project Start
1993-12-10
Project End
1998-11-30
Budget Start
1996-12-01
Budget End
1997-11-30
Support Year
4
Fiscal Year
1997
Total Cost
Indirect Cost
Name
Albert Einstein College of Medicine
Department
Microbiology/Immun/Virology
Type
Schools of Medicine
DUNS #
009095365
City
Bronx
State
NY
Country
United States
Zip Code
10461
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