Osteoarthritis (OA) affects over 50 million Americans and has a substantial impact on the economy and the health care system. Currently, there is no cure for this debilitating disease and the effective treatment is, at best, focused on symptomatic relief. Cartilage degeneration is thought to be the primary pathology associated with the disease. Recent developments in chondro-protective drugs, gene therapy, and the development of biomaterials for cartilage resurfacing have generated substantial demand for noninvasive techniques for detecting changes in cartilage. These developments and any other potential therapies can effectively work only if the disease is detected early. Among the most significant early changes in articular cartilage in OA is the loss of proteoglycans (PG). Conventional MRI techniques, which rely on the T1, T2, proton density, and magnetization transfer (MT) have proven inconclusive in the detection of early biochemical changes in the cartilage. However, these techniques are excellent for detecting the gross tissue morphological changes in the knee joint. A non-invasive assessment of biochemical markers in OA would have a major impact on the ability to make an early and appropriate therapeutic intervention, monitor the clinical outcome, and aid in evaluating new treatment modalities. Recently, we have demonstrated in model systems, ex-vivo and in-vivo pilot studies that the spin-lock MRI can track early biochemical changes in cartilage. In this proposal, we are employing the high-resolution proton T1? relaxation mapping in order to characterize various clinical grades of OA (natural disease) obtained from total knee replacement surgery patients and validating against reference standard sodium MRI. Secondly, we will optimize the high-resolution (spatial and temporal) 3D -T1? relaxation mapping with transmit-receive 8-channel phased array knee coil and parallel imaging (GRAPPA, m-SENSE) at 3T on healthy controls as well as symptomatic subjects in a temporal fashion (0, 6, 12 and 18 months) to determine whether the ex-vivo observations hold true in-vivo situations and correlate with delayed gadolinium enhanced MRI of cartilage (3D-dGEMRIC). The purpose of this proposal is to characterize and identify the role of high-resolution non-invasive MR methods that assess disease risk and progression. Specifically, we will assess whether identified early OA lesions at baseline T1?-MRI examinations are predictive of morphological loss of cartilage over an 18 month period. The novel non-invasive biochemical markers will not only affect our ability to characterize/monitor large OA population in a quantitative manner but also possibly identify patients at risk for rapid progression of disease. Successful completion of proposed work has the potential for defining early biochemical markers that are risk factors and predictors for knee OA progression.

National Institute of Health (NIH)
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Research Project (R01)
Project #
Application #
Study Section
Skeletal Biology Structure and Regeneration Study Section (SBSR)
Program Officer
Lester, Gayle E
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
New York University
Schools of Medicine
New York
United States
Zip Code
Chang, Gregory; Honig, Stephen; Liu, Yinxiao et al. (2015) 7 Tesla MRI of bone microarchitecture discriminates between women without and with fragility fractures who do not differ by bone mineral density. J Bone Miner Metab 33:285-93
Wang, Ligong; Chang, Gregory; Bencardino, Jenny et al. (2015) T1rho MRI at 3T of menisci in patients with acute anterior cruciate ligament (ACL) injury. J Magn Reson Imaging 41:544-9
Wang, Ligong; Regatte, Ravinder R (2015) Investigation of regional influence of magic-angle effect on t2 in human articular cartilage with osteoarthritis at 3 T. Acad Radiol 22:87-92
Madelin, Guillaume; Poidevin, Frederick; Makrymallis, Antonios et al. (2015) Classification of sodium MRI data of cartilage using machine learning. Magn Reson Med 74:1435-48
Wang, Ligong; Regatte, Ravinder R (2015) T?? MRI of human musculoskeletal system. J Magn Reson Imaging 41:586-600
Wang, Ligong; Salibi, Nouha; Chang, Gregory et al. (2014) Evaluation of subchondral bone marrow lipids of acute anterior cruciate ligament (ACL)-injured patients at 3 T. Acad Radiol 21:758-66
Wang, Ligong; Chang, Gregory; Bencardino, Jenny et al. (2014) T1rho MRI of menisci in patients with osteoarthritis at 3 Tesla: a preliminary study. J Magn Reson Imaging 40:588-95
Wang, Ligong; Regatte, Ravinder R (2014) Quantitative mapping of human cartilage at 3.0T: parallel changes in T?, T??, and dGEMRIC. Acad Radiol 21:463-71
Madelin, Guillaume; Lee, Jae-Seung; Regatte, Ravinder R et al. (2014) Sodium MRI: methods and applications. Prog Nucl Magn Reson Spectrosc 79:14-47
Madelin, Guillaume; Babb, James; Xia, Ding et al. (2013) Articular cartilage: evaluation with fluid-suppressed 7.0-T sodium MR imaging in subjects with and subjects without osteoarthritis. Radiology 268:481-91

Showing the most recent 10 out of 45 publications