Abstract

The overall goal of the proposed animal research is to provide new information relating in vivo 31P-Nuclear Magnetic Resonance (NMR) Spectroscopy to 11C or 18F-2-deoxyglucose (2DG) and 11C-Methionine Positron Emission Tomography (PET) Scanning in the evaluation of tumor metabolism during various stages of tumor growth in both untreated and treated (chemotherapy) animals.
More specific aims i nclude quantitating tumor high energy phosphate metabolites and pH using 31P-NMR Spectroscopy (both in vivo and in vitro), glucose uptake and amino acid uptake using PET Scanning, tumor protein synthesis and ATP levels using biochemical techniques and tumor morphology using computerized axial tomography (CT), NMR proton imaging and histology. The above parameters will be compared in two animal tumor models (Rabbit VX-2 hind limb muscle tumor and adult canine brain tumor) during various stages of tumor growth and following either intravenous or intraarterial chemotherapy. Results of the studies should provide important information relating to tumor biology and the potential clinical value of using in vivo imaging and spectroscopy techniques to monitor metabolic changes during the natural history of tumor growth and following therapy. The application of the results could provide a new approach to monitoring cancer therapy and determining prognosis.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA037870-02
Application #
3175763
Study Section
Diagnostic Radiology Study Section (RNM)
Project Start
1984-07-01
Project End
1987-06-30
Budget Start
1985-07-01
Budget End
1986-06-30
Support Year
2
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Johns Hopkins University
Department
Type
Schools of Medicine
DUNS #
045911138
City
Baltimore
State
MD
Country
United States
Zip Code
21218

  Publications

Conti, P S; Alauddin, M M; Fissekis, J R et al. (1995) Synthesis of 2'-fluoro-5-[11C]-methyl-1-beta-D-arabinofuranosyluracil ([11C]-FMAU): a potential nucleoside analog for in vivo study of cellular proliferation with PET. Nucl Med Biol 22:783-9
Alauddin, M M; Ravert, H T; Musachio, J L et al. (1995) Selective alkylation of pyrimidyl dianions III: no-carrier-added synthesis of [11C-methyl]-thymidine. Nucl Med Biol 22:791-4
Anderson, J H; Strandberg, J D; Wong, D F et al. (1994) Multimodality correlative study of canine brain tumors. Proton magnetic resonance spectroscopy, positron emission tomography, and histology. Invest Radiol 29:597-605
Barker, P B; Breiter, S N; Soher, B J et al. (1994) Quantitative proton spectroscopy of canine brain: in vivo and in vitro correlations. Magn Reson Med 32:157-63
Conti, P S; Hilton, J; Wong, D F et al. (1994) High performance liquid chromatography of carbon-11 labeled thymidine and its major catabolites for clinical PET studies. Nucl Med Biol 21:1045-51
Barker, P B; Blackband, S J; Chatham, J C et al. (1993) Quantitative proton spectroscopy and histology of a canine brain tumor model. Magn Reson Med 30:458-64
Wunderlich, C C; Enterline, J P; Anderson, J H (1992) Gravimetric measurements of cerebral edema in a rabbit brain tumor model. Neurosurgery 31:1079-83;discussion 1083-4
Wunderlich, C C; Janes, N J; Samphilipo Jr, M A et al. (1989) Renal perfusion model for NMR spectroscopy. Invest Radiol 24:619-25
Mills, P; Buonomo, C; Pillai, R P et al. (1988) In vivo and in vitro 31P-NMR preliminary studies of the VX-2 carcinoma in rabbits. Invest Radiol 23:584-91
Samphilipo Jr, M A; Hassenbusch, S J; Grochow, L B et al. (1987) A reservoir model for repeated CSF access in the rabbit. J Neurosci Methods 22:47-52

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