Abstract

The overall goal of our animal studies is to evaluate how various in vitro functional and morphological parameters compare with MRI/31p-MMR and PET data of brain tumor growth and response to steroid therapy. PET data will include blood flow, oxygen consumptionand 18F-2-Fluoro-2-deoxyglucose uptake. The studies are designed to quantitate data using in vivo MRI/MRS and PET techniques as well as established in vitro assay procedures. In the canine work, MRI and PET studies will be performed on the same animals using localization techniques that will provide examination of the same tissue sample volume. The results of both non invasive in vivo procedures will be correlated to in vitro detailed morphometric-histological analysis of the tumors as well as brain/ tumor tissue specific gravity measurements and blood flow determinations using microsphere techniques. High resolution (microscopy) imaging procedures will be employed to study rabbit brain tumors as a function of their growth and response to therapy aimed at reducing tumor induced edema. The MRI high resolution images will be compared to detailed histological evaluation of the sample volume area. Tumor associated edema will be quantitated using measurements of Tl and T2 relaxation times and compared to direct in vitro measurements of tumor and edematous brain tissue water content extravasation of plasma proteins and vascular - extravascular space compartment determinations. The proposed studies address timely and important issues relating to the information provided and the potential clinical applications of MRI/MRS and PET.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA037870-07
Application #
3175767
Study Section
Diagnostic Radiology Study Section (RNM)
Project Start
1984-07-01
Project End
1993-11-30
Budget Start
1991-12-01
Budget End
1992-11-30
Support Year
7
Fiscal Year
1992
Total Cost
Indirect Cost

  Institution

Name
Johns Hopkins University
Department
Type
Schools of Medicine
DUNS #
045911138
City
Baltimore
State
MD
Country
United States
Zip Code
21218

  Publications

Conti, P S; Alauddin, M M; Fissekis, J R et al. (1995) Synthesis of 2'-fluoro-5-[11C]-methyl-1-beta-D-arabinofuranosyluracil ([11C]-FMAU): a potential nucleoside analog for in vivo study of cellular proliferation with PET. Nucl Med Biol 22:783-9
Alauddin, M M; Ravert, H T; Musachio, J L et al. (1995) Selective alkylation of pyrimidyl dianions III: no-carrier-added synthesis of [11C-methyl]-thymidine. Nucl Med Biol 22:791-4
Anderson, J H; Strandberg, J D; Wong, D F et al. (1994) Multimodality correlative study of canine brain tumors. Proton magnetic resonance spectroscopy, positron emission tomography, and histology. Invest Radiol 29:597-605
Barker, P B; Breiter, S N; Soher, B J et al. (1994) Quantitative proton spectroscopy of canine brain: in vivo and in vitro correlations. Magn Reson Med 32:157-63
Conti, P S; Hilton, J; Wong, D F et al. (1994) High performance liquid chromatography of carbon-11 labeled thymidine and its major catabolites for clinical PET studies. Nucl Med Biol 21:1045-51
Barker, P B; Blackband, S J; Chatham, J C et al. (1993) Quantitative proton spectroscopy and histology of a canine brain tumor model. Magn Reson Med 30:458-64
Wunderlich, C C; Enterline, J P; Anderson, J H (1992) Gravimetric measurements of cerebral edema in a rabbit brain tumor model. Neurosurgery 31:1079-83;discussion 1083-4
Wunderlich, C C; Janes, N J; Samphilipo Jr, M A et al. (1989) Renal perfusion model for NMR spectroscopy. Invest Radiol 24:619-25
Mills, P; Buonomo, C; Pillai, R P et al. (1988) In vivo and in vitro 31P-NMR preliminary studies of the VX-2 carcinoma in rabbits. Invest Radiol 23:584-91
Samphilipo Jr, M A; Hassenbusch, S J; Grochow, L B et al. (1987) A reservoir model for repeated CSF access in the rabbit. J Neurosci Methods 22:47-52

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