Abstract

We have determined that gene sequences homologous to the DNA domain of SV40 large T antigen are present and expressed in uninfected, nontransformed cells. Expression of this gene appears to be cell growth- and, in fact, cell cycle-related. This application proposes experiments designed to elucidate in greater detail: (1) the extent of homology to large T antigen at both the nucleic acid and amino acid level; (2) the expression of this gene during the various parts of the G-1 stage of the cell cycle; and (3) the role it plays by itself and in conjunction with other cell division cycle genes in the fundamental processes of cell proliferation. Such studies will provide important information about the nature and temporal sequence of events involved in the progression of a mammalian cell from ?G?o through G1 to S and eventually on to mitosis. (N)

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
1R01CA040332-01
Application #
3180136
Study Section
Molecular Cytology Study Section (CTY)
Project Start
1985-07-01
Project End
1988-06-30
Budget Start
1985-07-01
Budget End
1986-06-30
Support Year
1
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Temple University
Department
Type
Schools of Medicine
DUNS #
City
Philadelphia
State
PA
Country
United States
Zip Code
19122

  Publications

Borrelli, M J; Stafford, D M; Rausch, C M et al. (1992) Cycloheximide protection against actinomycin D cytotoxicity. J Cell Physiol 153:507-17
Cosenza, S C; Carter, R; Pena, A et al. (1991) Growth-associated gene expression is not constant in cells traversing G-1 after exiting mitosis. J Cell Physiol 147:231-41
Whitman, M M; Shen, Y M; Soprano, D et al. (1990) Molecular analysis of early growth-associated events during the differentiation of F9 cells into embryoid bodies. Cancer Res 50:3193-8
Owen, T A; Carter, R; Whitman, M M et al. (1990) Evidence that density-dependent growth arrest is a two-stage process in WI-38 cells. J Cell Physiol 142:137-48
Toscani, A; Soprano, D R; Soprano, K J (1990) Sodium butyrate in combination with insulin or dexamethasone can terminally differentiate actively proliferating Swiss 3T3 cells into adipocytes. J Biol Chem 265:5722-30
Owen, T A; Soprano, D R; Soprano, K J (1989) Analysis of the growth factor requirements for stimulation of WI-38 cells after extended periods of density-dependent growth arrest. J Cell Physiol 139:424-31
Owen, T A; Cosenza, S C; Soprano, D R et al. (1989) Evidence that stimulation of growth following long term density arrest of WI-38 cells proceeds via a pathway independent of protein kinase C and of cAMP-dependent protein kinase. Oncogene Res 4:137-47
Toscani, A; Soprano, D R; Soprano, K J (1988) Molecular analysis of sodium butyrate-induced growth arrest. Oncogene Res 3:223-38
Soprano, D R; Soprano, K J; Wyatt, M L et al. (1988) Induction of the expression of retinol-binding protein and transthyretin in F9 embryonal carcinoma cells differentiated to embryoid bodies. J Biol Chem 263:17897-900
Gimmi, E R; Soprano, K J; Rosenberg, M et al. (1988) Deletions in the SV40 late polyadenylation region downstream of the AATAAA mediate similar effects on expression in various mammalian cell lines. Nucleic Acids Res 16:8977-97

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