We have determined that gene sequences homologous to the DNA domain of SV40 large T antigen are present and expressed in uninfected, nontransformed cells. Expression of this gene appears to be cell growth- and, in fact, cell cycle-related. This application proposes experiments designed to elucidate in greater detail: (1) the extent of homology to large T antigen at both the nucleic acid and amino acid level; (2) the expression of this gene during the various parts of the G-1 stage of the cell cycle; and (3) the role it plays by itself and in conjunction with other cell division cycle genes in the fundamental processes of cell proliferation. Such studies will provide important information about the nature and temporal sequence of events involved in the progression of a mammalian cell from ?G?o through G1 to S and eventually on to mitosis. (N)

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA040332-03
Application #
3180142
Study Section
Molecular Cytology Study Section (CTY)
Project Start
1985-07-01
Project End
1989-06-30
Budget Start
1987-07-01
Budget End
1989-06-30
Support Year
3
Fiscal Year
1987
Total Cost
Indirect Cost
Name
Temple University
Department
Type
Schools of Medicine
DUNS #
City
Philadelphia
State
PA
Country
United States
Zip Code
19122
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