Abstract

The long term goals of this research are to provide evidence for and an understanding of the role of human papilloma viruses in the development of cervical dysplasias and invasive carcinomas.
The specific aims i nclude: 1) Establish a correlation between HPV types DNA ploidy patterns and morphometric parameters seen in histologic sections. 2) a) Characterize the state of viral DNA. b) Map viral transcripts. c) Identify """"""""early"""""""" viral proteins and elucidate their function in cervical carcinomas and carcinoma derived cell lines. 3) Investigate aspects of the immune response in papillomavirus infections. a) Determine the presence of antibodies to """"""""early"""""""" viral proteins. b) Attempt to construct type specific antigens for """"""""oncogenic"""""""" HPV""""""""s and search for antibodies to these antigens in human sera which may provide an indication about the spread of these viruses in the human population.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
1R01CA042126-01
Application #
3182983
Study Section
(SSS)
Project Start
1986-04-01
Project End
1989-03-31
Budget Start
1986-04-01
Budget End
1987-03-31
Support Year
1
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
University of California Los Angeles
Department
Type
Schools of Medicine
DUNS #
119132785
City
Los Angeles
State
CA
Country
United States
Zip Code
90095

  Publications

Gage, J R; Meyers, C; Wettstein, F O (1990) The E7 proteins of the nononcogenic human papillomavirus type 6b (HPV-6b) and of the oncogenic HPV-16 differ in retinoblastoma protein binding and other properties. J Virol 64:723-30
Fu, Y S; Cheng, L; Huang, I et al. (1989) DNA ploidy analysis of cervical condyloma and intraepithelial neoplasia in specimens obtained by punch biopsy. Anal Quant Cytol Histol 11:187-95
Gupta, J W; Saito, K; Saito, A et al. (1989) Human papillomaviruses and the pathogenesis of cervical neoplasia. A study by in situ hybridization. Cancer 64:2104-10
Smotkin, D; Prokoph, H; Wettstein, F O (1989) Oncogenic and nononcogenic human genital papillomaviruses generate the E7 mRNA by different mechanisms. J Virol 63:1441-7
Crissman, J D; Kessis, T; Shah, K V et al. (1988) Squamous papillary neoplasia of the adult upper aerodigestive tract. Hum Pathol 19:1387-96
Fu, Y S; Huang, I; Beaudenon, S et al. (1988) Correlative study of human papillomavirus DNA, histopathology and morphometry in cervical condyloma and intraepithelial neoplasia. Int J Gynecol Pathol 7:297-307
Shibata, D; Fu, Y S; Gupta, J W et al. (1988) Detection of human papillomavirus in normal and dysplastic tissue by the polymerase chain reaction. Lab Invest 59:555-9
Saito, K; Saito, A; Fu, Y S et al. (1987) Topographic study of cervical condyloma and intraepithelial neoplasia. Cancer 59:2064-70
Smotkin, D; Wettstein, F O (1987) The major human papillomavirus protein in cervical cancers is a cytoplasmic phosphoprotein. J Virol 61:1686-9
Saito, K; Saito, A; Fu, Y S et al. (1986) Immunoreactivity of involucrin in cervical condyloma and intraepithelial neoplasia. Int J Gynecol Pathol 5:308-18