Abstract

The development of leukemia in hemocytes of the soft-shell clam presents a non-mammalian model system in which to study the expression of normal and tumor- specific proteins. The proposed work focuses on recently discovered proteins expressed by normal and leukemic hemocytes. Protein sequence data will be obtained, and a cDNA library made from leukemic cells will be screened with monoclonal and polyclonal antibodies made to the a 150 kD protein expressed predominantly in leukemic cells, as well as a 130 kD protein expressed predominantly in normal hemocytes. Sequences obtained will then be compared to known genes in order to obtain information on the function of these gene products. Studies will be carried out to localize the expression of these proteins following sub- cellular fractionation. In addition, if the genes encoding these proteins can be isolated, analysis of expression in clam tissues will be carried out using nucleic acid probes. These proposed studies will be carried out using FACS analysis, production of murine monoclonal antibodies, protein sequencing, cDNA cloning, Western, Southern, and Northern blotting, and fluorescence and confocal microscopy.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
2R01CA044307-04A2
Application #
3186868
Study Section
Experimental Immunology Study Section (EI)
Project Start
1987-08-10
Project End
1996-02-28
Budget Start
1993-02-19
Budget End
1994-02-28
Support Year
4
Fiscal Year
1993
Total Cost
Indirect Cost

  Institution

Name
Tufts University
Department
Type
Schools of Veterinary Medicine
DUNS #
604483045
City
Boston
State
MA
Country
United States
Zip Code
02111

  Publications

Stephens, R E; Walker, C W; Reinisch, C L (2001) Multiple protein differences distinguish clam leukemia cells from normal hemocytes: evidence for the involvement of p53 homologues. Comp Biochem Physiol C Toxicol Pharmacol 129:329-38
Kreiling, J A; Jessen-Eller, K; Miller, J et al. (2001) Early development of the serotonergic and dopaminergic nervous system in Spisula solidissima (surf clam) larvae. Comp Biochem Physiol A Mol Integr Physiol 130:341-51
Kelley, M L; Winge, P; Heaney, J D et al. (2001) Expression of homologues for p53 and p73 in the softshell clam (Mya arenaria), a naturally-occurring model for human cancer. Oncogene 20:748-58
Kreiling, J A; Stephens, R E; Kuzirian, A M et al. (2000) Polychlorinated biphenyls are selectively neurotoxic in the developing Spisula solidissima embryo. J Toxicol Environ Health A 61:657-75
Smith, C R; Barker, C M; Barker, L F et al. (1999) Polychlorinated biphenyls (PCBs) selectively disrupt serotonergic cell growth in the developing Spisula embryo. Toxicol Sci 50:54-63
Jessen-Eller, K; Steele, M; Reinisch, C et al. (1998) Blockade of ryanodine receptors stimulates neurite outgrowth in embryos of Spisula solidissima. Biol Bull 195:206-7
Barker, C M; Calvert, R J; Walker, C W et al. (1997) Detection of mutant p53 in clam leukemia cells. Exp Cell Res 232:240-5
Harper, D M; Flessas, D A; Reinisch, C L (1994) Specific reactivity of leukemia cells to polyclonal anti-PCB antibodies. J Invertebr Pathol 64:234-7
White, M K; Miosky, D; Flessas, D A et al. (1993) The expression of an adhesion-related protein by clam hemocytes. J Invertebr Pathol 61:253-9
Smolowitz, R M; Reinisch, C L (1993) A novel adhesion protein expressed by ciliated epithelium, hemocytes, and leukemia cells in soft-shell clams. Dev Comp Immunol 17:475-81

Showing the most recent 10 out of 13 publications