The mechanisms by which carcinogenic chemicals and oncogenic viruses can transform proximal tubule cells of the kidney are largely unknown. Since the proximal tubule epithelial cells are the progenitors of most renal neoplasms, it is important to define their growth and differentiation programs in order to understand renal cancer. We will design a model to study renal carcinogenesis using pure rat kidney proximal tubule cells (RPTE) in culture. RPTE will be established in culture using selective techniques to insure a pure population. Cells will be transformed in vitro by known renal carcinogens and DNA-tumor viruses. Selection techniques for isolating transformed cells will be used both to quantitate the frequency of transformation and to prepare cells for further studies on the mechanism of transformation. The model will be used to determine the transformation potential of a novel group of toxins, S-cysteine conjugates, which may be the carcinogenic species produced from some xenobiotics. The growth factor requirements of transformed cells will be determined. These studies will provide a much needed data base for the investigation of renal carcinogenesis and the transformation of proximal tubule epithelial cells. In the future, the model will be extended into human studies.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA048197-02
Application #
3192251
Study Section
Chemical Pathology Study Section (CPA)
Project Start
1988-07-01
Project End
1990-04-30
Budget Start
1989-05-01
Budget End
1990-04-30
Support Year
2
Fiscal Year
1989
Total Cost
Indirect Cost
Name
Adirondack Biomedical Research Institute
Department
Type
DUNS #
City
Lake Placid
State
NY
Country
United States
Zip Code
12946