Abstract

Kaposi's sarcoma associated herpesvirus (KSHV) or human herpesvirus 8 (HHV-8) was found in 100% of Kaposi's sarcoma and many body cavity-based lymphomas. It has been hypothesized that KSHV causes these malignancies. KSHV DNA can persist in a latent episomal form and a linear replicative form. The episomal form has been shown to be maintained by the cellular DNA polymerase. It is hypothesized that the episomal viral DNA is maintained by a specific viral origin of replication (ori P) and a DNA binding protein termed trans-activator which is involved in replication of the viral episome.
Three aims are proposed. The first is to identify ori P of KSHV. The second is to map the initiation site and direction of replication.
The third aim i s to search for the transactivator. This study should lead to better understanding of latency.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA075895-03
Application #
2896218
Study Section
AIDS and Related Research Study Section 3 (ARRC)
Program Officer
Read-Connole, Elizabeth Lee
Project Start
1997-08-01
Project End
2001-05-31
Budget Start
1999-06-01
Budget End
2000-05-31
Support Year
3
Fiscal Year
1999
Total Cost
Indirect Cost

  Institution

Name
University of South Florida
Department
Biochemistry
Type
Schools of Medicine
DUNS #
City
Tampa
State
FL
Country
United States
Zip Code
33612