Abstract

Our role in this collaboration will be the construction and biological characterization of AT6.1-12 microcell hybrids that contain the HOXC gene locus. The Miller laboratory will then analyze HOXC gene expression patterns in these hybrids to see if there is a correlation between HOXC gene expression and in vivo biological activity. Once candidate HOXC genes have been identified, our laboratory will test their potential biological activity by transfecting them into AT6.1 cells, establishing appropriate transgene and vector only controls, and finally testing these cell lines in vivo.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA084269-04
Application #
6626734
Study Section
Special Emphasis Panel (ZRG1-MEP (01))
Program Officer
Spalholz, Barbara A
Project Start
2000-01-01
Project End
2005-12-31
Budget Start
2003-01-01
Budget End
2005-12-31
Support Year
4
Fiscal Year
2003
Total Cost
$352,771
Indirect Cost

  Institution

Name
University of Colorado Denver
Department
Pathology
Type
Schools of Medicine
DUNS #
041096314
City
Aurora
State
CO
Country
United States
Zip Code
80045

  Publications

Axlund, Sunshine Daddario; Lambert, James R; Nordeen, Steven K (2010) HOXC8 inhibits androgen receptor signaling in human prostate cancer cells by inhibiting SRC-3 recruitment to direct androgen target genes. Mol Cancer Res 8:1643-55
Miller, Gary J; Miller, Heidi L; van Bokhoven, Adrie et al. (2003) Aberrant HOXC expression accompanies the malignant phenotype in human prostate. Cancer Res 63:5879-88
van Bokhoven, Adrie; Varella-Garcia, Marileila; Korch, Christopher et al. (2003) Molecular characterization of human prostate carcinoma cell lines. Prostate 57:205-25
van Bokhoven, Adrie; Caires, Aimee; Maria, Michael Di et al. (2003) Spectral karyotype (SKY) analysis of human prostate carcinoma cell lines. Prostate 57:226-44
van Bokhoven, A; Varella-Garcia, M; Korch, C et al. (2001) Widely used prostate carcinoma cell lines share common origins. Prostate 47:36-51
Varella-Garcia, M; Boomer, T; Miller, G J (2001) Karyotypic similarity identified by multiplex-FISH relates four prostate adenocarcinoma cell lines: PC-3, PPC-1, ALVA-31, and ALVA-41. Genes Chromosomes Cancer 31:303-15
van Bokhoven, A; Varella-Garcia, M; Korch, C et al. (2001) TSU-Pr1 and JCA-1 cells are derivatives of T24 bladder carcinoma cells and are not of prostatic origin. Cancer Res 61:6340-4
Golpon, H A; Geraci, M W; Moore, M D et al. (2001) HOX genes in human lung: altered expression in primary pulmonary hypertension and emphysema. Am J Pathol 158:955-66