Abstract

Research will be done in two areas: Dextran Biosynthesis: 1. We will purify and characterize the enzymes for Strep. mutans which are involved in the synthesis of water soluble and water-insoluble dextrans. 2. Studies will be made of the role of potential primers in the synthesis reaction and efforts will be directed at developing effective enzymic inhibitors. Microtubule Chemistry: 1. The mechanism for presteady state and steady state microtubule assembly will be studied. 2. The role of microtubule-associated proteins in the regulation of microtubule assembly will be analyzed.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Research Project (R01)
Project #
5R01DE003246-16
Application #
3218837
Study Section
Cellular Biology and Physiology Subcommittee 1 (CBY)
Project Start
1976-06-01
Project End
1986-05-31
Budget Start
1985-06-01
Budget End
1986-05-31
Support Year
16
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University of North Carolina Chapel Hill
Department
Type
Schools of Dentistry/Oral Hygn
DUNS #
078861598
City
Chapel Hill
State
NC
Country
United States
Zip Code
27599

  Publications

Caplow, M; Shanks, J (1990) Mechanism of the microtubule GTPase reaction. J Biol Chem 265:8935-41
Caplow, M; Shanks, J (1990) Mechanism for oscillatory assembly of microtubules. J Biol Chem 265:1414-8
Caplow, M; Ruhlen, R; Shanks, J et al. (1989) Stabilization of microtubules by tubulin-GDP-Pi subunits. Biochemistry 28:8136-41
Caplow, M; Shanks, J; Breidenbach, S et al. (1988) Kinetics and mechanism of microtubule length changes by dynamic instability. J Biol Chem 263:10943-51
Caplow, M; Shanks, J; Ruhlen, R L (1988) Temperature-jump studies of microtubule dynamic instability. J Biol Chem 263:10344-52
Caplow, M (1986) Location of the guanosine triphosphate (GTP) hydrolysis site in microtubules. Ann N Y Acad Sci 466:510-8
Caplow, M; Shanks, J; Brylawski, B P (1986) Differentiation between dynamic instability and end-to-end annealing models for length changes of steady-state microtubules. J Biol Chem 261:16233-40
Caplow, M; Reid, R (1985) Directed elongation model for microtubule GTP hydrolysis. Proc Natl Acad Sci U S A 82:3267-71
Caplow, M; Shanks, J; Brylawski, B P (1985) Concerning the location of the GTP hydrolysis site on microtubules. Can J Biochem Cell Biol 63:422-9
Caplow, M; Shanks, J; Brylawski, B P (1985) Concerning the anomalous kinetic behavior of microtubules. J Biol Chem 260:12675-9