The long-term objective of this grant is to study the microbial composition of developing dental plaque. A predominant cultural study will compare the microbiota of sites with no attachment loss and sites with the earliest detectable lesions. Suspected periodontal pathogens will be sought in deeper pockets of the same oral cavity to determine whether early lesions are an extension of an existing infection or represent a new (different) infection. Screening for, and directing treatment against, species related to initial changes could prevent periodontal destruction. A second study will seek to determine if local periodontal therapy can change the microbiota and eliminate suspected periodontal pathogens from early lesions. Plaque development, with continued presence of pathogens and persisting disease will suggest that alternate, more effective treatments will be necessary. The third specific aim concentrates on the development of methods to identify microorganisms in plaque samples. Definition of the microbiota of a site requires knowledge of the species present and sensitive methods to identify them.
This specific aim will investigate methods to optimize use of polyacrylamide gel electrophoresis (SDS-PAGE) of total cell proteins so that pure cultures from periodontal pockets can be identified accurately and rapidly. If unidentifiable species are found, a combination of biochemical features, SDS-PAGE and DNA/DNA hybridizations will be used to define the taxonomic groups of gram negative and positive rods. This will greatly help investigators describe the microbiota related to periodontal health and disease.

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Research Project (R01)
Project #
5R01DE003488-18
Application #
3218871
Study Section
Oral Biology and Medicine Study Section (OBM)
Project Start
1976-02-01
Project End
1991-06-30
Budget Start
1989-07-01
Budget End
1990-06-30
Support Year
18
Fiscal Year
1989
Total Cost
Indirect Cost
Name
Forsyth Institute
Department
Type
DUNS #
City
Cambridge
State
MA
Country
United States
Zip Code
02142
Tanner, A; Kent, R; Maiden, M F et al. (1996) Clinical, microbiological and immunological profile of healthy, gingivitis and putative active periodontal subjects. J Periodontal Res 31:195-204
Maiden, M F; Tanner, A; Moore, W E (1992) Identification of Selenomonas species by whole-genomic DNA probes, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, biochemical tests and cellular fatty acid analysis. Oral Microbiol Immunol 7:7-13
Maiden, M F; Tanner, A (1991) Identification of oral yeasts by polyacrylamide gel electrophoresis. Oral Microbiol Immunol 6:187-90
Goodson, J M; Tanner, A; McArdle, S et al. (1991) Multicenter evaluation of tetracycline fiber therapy. III. Microbiological response. J Periodontal Res 26:440-51
Maiden, M F; Tanner, A; McArdle, S et al. (1991) Tetracycline fiber therapy monitored by DNA probe and cultural methods. J Periodontal Res 26:452-9
Tanner, A; Bouldin, H (1989) The microbiota of early periodontitis lesions in adults. J Clin Periodontol 16:467-71
Tanner, A; Bouldin, H D; Maiden, M F (1989) Newly delineated periodontal pathogens with special reference to selenomonas species. Infection 17:182-7
Tanner, A C; Dzink, J L; Ebersole, J L et al. (1987) Wolinella recta, campylobacter concisus, bacteroides gracilis, and Eikenella corrodens from periodontal lesions. J Periodontal Res 22:327-30
Tanner, A C; Dzink, J L; Socransky, S S et al. (1987) Diagnosis of periodontal disease using rapid identification of ""activity-related"" gram-negative species. J Periodontal Res 22:207-8
Tanner, A (1987) Media for cultivation of Eikenella corrodens and formate-and fumarate-requiring species of oral bacteria. Oral Microbiol Immunol 2:187-9

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