1. Interactions of Neuropeptides on Acetylcholine (ACh) Release: Neuropeptides (calcitonin-gene related peptides, neuropeptide Y, tachykinins, vasoactive intestinal peptide/peptide histidine- isoleucine, bombesin/gastrin-releasing peptide and cholecystokinin-octapeptide) will be examined on myenteric and submucous plexus in terms of their ability to modulate release of ACh from cholinergic neurons. ACh will be measured by radiochemical and radioenzymatic methods. 2. Characterization of Galanin Inhibitory Action on Enteric Cholinergic Neurons: Inhibition by galanin of basal and neuro- peptide-evoked ACh release from myenteric plexus, submucous plexus neurons and from enzyme-dissociated myenteric ganglia will be examined. Comparisons will be made with inhibition of ACh release by somatostatin and opioid peptides. 3. Mechanism of Release of Substance P from Enteric Neurons: Basal, neuropeptide- and field-stimulated release of substance P from tissue preparations mentioned in Aim #2 will be determined by an enzyme-linked immunosorbent assay using a monoclonal anti-substance P antibody. 4. Characterization of Neuropeptide Receptors on GABAergic Neurons: Release of GABA by neuropeptide stimulation from enteric neurons will be measured by a radiochemical method. Specific antagonists will be used to characterize neuropeptide receptor sites on GABAergic neurons.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
5R01DK026860-08
Application #
3228072
Study Section
General Medicine A Subcommittee 2 (GMA)
Project Start
1981-04-01
Project End
1991-03-31
Budget Start
1988-04-01
Budget End
1989-03-31
Support Year
8
Fiscal Year
1988
Total Cost
Indirect Cost
Name
Southern Illinois University Carbondale
Department
Type
Schools of Medicine
DUNS #
939007555
City
Carbondale
State
IL
Country
United States
Zip Code
62901
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Yau, W M; Bowen, D J; Youther, M L (1991) Evidence for an involvement of eicosanoids in neurokinin3-receptor mediated acetylcholine release from myenteric neurons. Neurosci Lett 129:259-61
Yau, W M (1990) Mode of stimulation of gallbladder contraction by substance K. Am J Physiol 259:G838-41
Yau, W M; Dorsett, J A; Youther, M L (1990) Modulation of submucosal cholinergic neurons by 5-hydroxytryptamine and neuropeptides. Am J Physiol 259:G1019-24