The studies designed in this proposal will examine two main areas in transport physiology: The mechanism whereby calcium is transported across the turtle bladder membrane, and the role of calcium and PTH on toad bladder transport. In turtle bladder we will examine the calcium transporting properties of the mitochondrial rich (MR) and granular (G) cells (membrane sodium NA-Ca exchange and Ca-ATPase activity). We postulate that the MR Cell contains high Ca-ATPase activity. From unidirectional calcium flux studies we further postulate that the turtle bladder has a Na-Ca exchanger on the apical membrane. Apical and basolateral vesicles will be isolated and tested for activity; if the Golgi membrane is the precursor of the apical membrane in turtle bladder as it is in toad, Golgi membranes should also exhibit Na-Ca exchange activity. In toad bladder PTH decreases membrane transport independent of cyclic AMP; PTH increases extracellular calcium uptake in isolated epithelial cells. Calcium transport in MR and G Cells will be studied in the presence and absence of PTH. We postulate that the effect of PTH on membrane transport, is not receptor mediated, and that the hormone will have no direct effect of Ca- ATPase activity or NA-Ca exchange. PTH may exert an indirect effect solely due to the increase in cell calcium. The role of PTH on cytosolic phospholipid exchange proteins will be examined as PTH may enhance their activity if extracellular calcium is not rate limiting.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
5R01DK036119-06
Application #
3234467
Study Section
General Medicine B Study Section (GMB)
Project Start
1985-01-01
Project End
1992-12-31
Budget Start
1990-01-01
Budget End
1990-12-31
Support Year
6
Fiscal Year
1990
Total Cost
Indirect Cost
Name
Texas Tech University
Department
Type
Schools of Medicine
DUNS #
609980727
City
Lubbock
State
TX
Country
United States
Zip Code
79430
Fiala, Kevin A; Brown, Jessica A; Ling, Hong et al. (2007) Mechanism of template-independent nucleotide incorporation catalyzed by a template-dependent DNA polymerase. J Mol Biol 365:590-602
Yang, Wei (2007) Human MutLalpha: the jack of all trades in MMR is also an endonuclease. DNA Repair (Amst) 6:135-9
Lee, Jae Young; Yang, Wei (2006) UvrD helicase unwinds DNA one base pair at a time by a two-part power stroke. Cell 127:1349-60
Eiam-ong, S; Kurtzman, N A; Sabatini, S (1996) Renal ATPases twenty-four hours after uninephrectomy: the role of IGF-1. Miner Electrolyte Metab 22:234-41
Sabatini, S (1996) The cellular basis of metabolic alkalosis. Kidney Int 49:906-17
Sabatini, S; Kurtzman, N A; Spohn, M (1995) Effects of pH on calcium transport in turtle bladder. Am J Kidney Dis 26:866-72
Hayward-Lester, A; Oefner, P J; Sabatini, S et al. (1995) Accurate and absolute quantitative measurement of gene expression by single-tube RT-PCR and HPLC. Genome Res 5:494-99
Eiam-Ong, S; Laski, M E; Kurtzman, N A (1995) Diseases of renal adenosine triphosphatase. Am J Med Sci 309:13-25
Cruz-Caudillo, J C; Sabatini, S (1995) Diabetic hyperosmolar syndrome. Nephron 69:201-10
Eiam-ong, S; Spohn, M; Kurtzman, N A et al. (1995) Insights into the biochemical mechanism of maleic acid-induced Fanconi syndrome. Kidney Int 48:1542-8

Showing the most recent 10 out of 43 publications